FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78973
Sequences flagged as poor quality0
Sequence length239
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC1491818.890000379876668No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC1179014.929153001658795No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC831810.532713712281412No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC69688.823268712091474No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC60907.711496334190167No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC47466.0096488673344055No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC35774.529396122725489No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC27643.499930355944437No Hit
GTGTCAGCAGCCGCGGTAATACGTAGAAGACTAGTGTTAGTCATCTTTAT21432.7135856558570652No Hit
GTGTCAGCCGCCGCGGTAATACGTAGAAGACTAGTGTTAGTCATCTTTAT17342.1956871335772985No Hit
GTGCCAGCAGCCGCGGTAATACGTAGAAGACTAGTGTTAGTCATCTTTAT11881.5043116001671457No Hit
GTGCCAGCCGCCGCGGTAATACGTAGAAGACTAGTGTTAGTCATCTTTAT10061.273853089030428No Hit
GTGTCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT9961.2611905334734657No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT9771.2371316779152368No Hit
GTGTCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT7941.005406911222823No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT7500.9496916667721879No Hit
GTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT5980.7572208223063579No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT5680.7192331556354703No Hit
GTGCCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT4930.6242639889582515No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT4700.5951401111772379No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTGTCCGGAATTAC1580.20006837780000758No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC1220.15448317779494258No Hit
GTGTCAGCAGCCGCGGTAATACCTAGGTCGCAAGCGTTATCCGGAATTAT1180.14941815557215757No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTGTCCGGAATTAC1150.14561938890506881No Hit
GTGTCAGCCGCCGCGGTAATACGTAGAAGACTAGTGTTAGTCCTCTTTAT1000.12662555556962507No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAT990.1253593000139288No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC940.11902802223544755No Hit
GTGTCAGCAGCCGCGGTAATACGTAGAAGACTAGTGTTAGTCCTCTTTAT920.11649551112405505No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTGTCCGGAATTAC830.10509921112278879No Hit
GTGTCAGCAGCCGCGGTAATACGTAGAAGCCTAGTGTTAGTCATCTTTAT790.10003418890000379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)