FastQCFastQC Report
Wed 8 May 2024
EGAF00004969492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39440
Total Bases9.4 Mbp
Sequences flagged as poor quality0
Sequence length239
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC455611.551724137931034No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC32488.235294117647058No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG26896.817951318458418No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC24996.336206896551724No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG20105.096348884381339No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC19304.893509127789047No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG14843.7626774847870186No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG11712.9690669371196754No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC9412.385902636916836No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT8952.2692697768762677No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC7431.8838742393509127No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT6171.5644016227180528No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC5771.4629817444219069No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT4541.1511156186612577No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC3981.0091277890466532No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGC3870.981237322515213No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGC3620.9178498985801217No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT3370.8544624746450304No Hit
GTGTCAGCAGCCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCG2410.6110547667342799No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCCATAGCATATGTTAAAGTTGC2290.5806288032454361No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT2230.5654158215010142No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGC2210.5603448275862069No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGC2170.5502028397565922No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCACTAGCGTATATTAAAGTTGC2080.5273833671399595No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGC2010.5096348884381339No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGC2000.5070993914807302No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGC1990.5045638945233266No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG1990.5045638945233266No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG1720.43610547667342797No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT1700.43103448275862066No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCCATAGCATATGTTAAAGTTGC1650.4183569979716024No Hit
GTGTCAGCCGCCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCG1590.40314401622718055No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCACTAGCGTATATTAAAGTTGC1570.39807302231237324No Hit
GTGCCAGCAGCCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCG1500.3803245436105477No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGC1460.37018255578093306No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGC1370.3473630831643002No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGC1330.3372210953346856No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG1320.33468559837728196No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC1230.3118661257606491No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT1200.3042596348884381No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCACTAGCGTATATTAAAGTTGC1160.29411764705882354No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCCATAGCATATGTTAAAGTTGC1150.29158215010141986No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG1140.28904665314401623No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTCTATTAAAGTTGC1130.28651115618661255No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT1040.26369168356997974No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGC970.24594320486815416No Hit
GTGCCAGCCGCCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCG950.24087221095334685No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC930.23580121703853954No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT930.23580121703853954No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG900.2281947261663286No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCCATAGCATATGTTAAAGTTGC850.21551724137931033No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGC740.1876267748478702No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCACTAGCGTATATTAAAGTTGC740.1876267748478702No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTCTATTAAAGTTGC730.18509127789046653No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT730.18509127789046653No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT700.17748478701825557No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTCTATTAAAGTTGC680.1724137931034483No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG580.14705882352941177No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC580.14705882352941177No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGT530.1343813387423935No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT520.13184584178498987No Hit
GTGTCAGCAGCCGCGGTAATTCCCGCTCCAATAGCGTATATTAAAGTTGC510.12931034482758622No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT510.12931034482758622No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT500.12677484787018256No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGT480.12170385395537525No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG480.12170385395537525No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTCTATTAAAGTTGC470.1191683569979716No Hit
GTGTCAGCAGCCGCGGTAACACGATTCACCCAAGTCAATAGAAGCCGGCG430.10902636916835699No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC420.10649087221095335No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGT410.1039553752535497No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT400.10141987829614604No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)