FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39440
Sequences flagged as poor quality0
Sequence length239
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTTTCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA13553.4355983772819476No Hit
GGACTACAAGGGTTTCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA11302.865111561866126No Hit
GGACTACTCGGGTTTCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA10862.7535496957403653No Hit
GGACTACTGGGGTTTCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA9562.4239350912778903No Hit
GGACTACCAGGGTTTCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA8952.2692697768762677No Hit
GGACTACTAGGGTATCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA8702.2058823529411766No Hit
GGACTACGAGGGTTTCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA7651.9396551724137931No Hit
GGACTACAGGGGTTTCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA7621.9320486815415823No Hit
GGACTACACGGGTTTCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA7531.9092292089249494No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC7311.853448275862069No Hit
GGACTACTCGGGTATCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA7121.8052738336713996No Hit
GGACTACTGGGGTATCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA6541.658215010141988No Hit
GGACTACGGGGGTTTCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA6491.6455375253549696No Hit
GGACTACAAGGGTATCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA6371.6151115618661258No Hit
GGACTACCCGGGTTTCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA6101.5466531440162272No Hit
GGACTACGCGGGTTTCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA5421.3742393509127788No Hit
GGACTACCAGGGTATCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA5361.359026369168357No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC5351.3564908722109534No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC5291.3412778904665315No Hit
GGACTACCGGGGTTTCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA5221.3235294117647058No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC5171.3108519269776877No Hit
GGACTACACGGGTATCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA4961.2576064908722109No Hit
GGACTACAGGGGTATCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA4801.2170385395537524No Hit
GGACTACGAGGGTATCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA4771.2094320486815417No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4301.09026369168357No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4251.0775862068965518No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4091.0370182555780934No Hit
GGACTACGGGGGTATCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA3991.0116632860040569No Hit
GGACTACCGGGGTATCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA3860.9787018255578093No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3780.9584178498985801No Hit
GGACTACCCGGGTATCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA3730.9457403651115619No Hit
GGACTACGAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3560.9026369168356998No Hit
GGACTACGCGGGTATCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA3500.8874239350912779No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3430.8696754563894524No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3410.8646044624746451No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA3050.7733265720081136No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2990.7581135902636916No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2950.7479716024340771No Hit
GGACTACGGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2780.704868154158215No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2750.697261663286004No Hit
GGACTACGCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2540.6440162271805274No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2530.6414807302231237No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2420.6135902636916836No Hit
GGACTACGAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2420.6135902636916836No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2260.5730223123732251No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2140.5425963488843814No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2120.537525354969574No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2060.5223123732251521No Hit
GGACTACGGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2010.5096348884381339No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1810.45892494929006084No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1690.42849898580121704No Hit
GGACTACGCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1570.39807302231237324No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1550.39300202839756593No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1540.39046653144016225No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1490.377789046653144No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1410.3575050709939148No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1400.35496957403651114No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1380.34989858012170383No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1380.34989858012170383No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1290.32707910750507097No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1290.32707910750507097No Hit
GGACTACTAGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1280.32454361054766734No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1200.3042596348884381No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1160.29411764705882354No Hit
GGACTACTCGGGTTTCTAATCGTTTTCGATATCCTAACCTTCGTTCTTGA1020.25862068965517243No Hit
GGACTACTAGGGTTTCTAATCGTTTTCGATATCCTAACCTTCGTTCTTGA1020.25862068965517243No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1010.25608519269776875No Hit
GGACTACAAGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA990.25101419878296144No Hit
GGACTACTGGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA950.24087221095334685No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA930.23580121703853954No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA910.23073022312373226No Hit
GGACTACTGGGGTTTCTAATCGTTTTCGATATCCTAACCTTCGTTCTTGA910.23073022312373226No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA860.21805273833671399No Hit
GGACTACTCGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA860.21805273833671399No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA820.2079107505070994No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA800.2028397565922921No Hit
GGACTACCAGGGTTTCTAATCGTTTTCGATATCCTAACCTTCGTTCTTGA800.2028397565922921No Hit
GGACTACTAGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA780.19776876267748478No Hit
GGACTACAAGGGTTTCTAATCGTTTTCGATATCCTAACCTTCGTTCTTGA770.19523326572008112No Hit
GGACTACCAGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA760.19269776876267747No Hit
GGACTACGAGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA740.1876267748478702No Hit
GGACTACTAGGGTATCTAATCGTTTTCGATATCCTAACCTTCGTTCTTGA720.18255578093306288No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA700.17748478701825557No Hit
GGACTACACGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA700.17748478701825557No Hit
GGACTACAGGGGTTTCTAATCGTTTTCGATATCCTAACCTTCGTTCTTGA700.17748478701825557No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTCGCTATTGTGTGTTC690.17494929006085191No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA680.1724137931034483No Hit
GGACTACACGGGTTTCTAATCGTTTTCGATATCCTAACCTTCGTTCTTGA670.16987829614604463No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA650.16480730223123732No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTCGCTATTGTGTGTTC630.15973630831643001No Hit
GGACTACAGGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA620.15720081135902636No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA600.15212981744421905No Hit
GGACTACGCGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA600.15212981744421905No Hit
GGACTACTGGGGTATCTAATCGTTTTCGATATCCTAACCTTCGTTCTTGA580.14705882352941177No Hit
GGACTACTCGGGTATCTAATCGTTTTCGATATCCTAACCTTCGTTCTTGA580.14705882352941177No Hit
GGACTACCCGGGTTTCTAATCGTTTTCGATATCCTAACCTTCGTTCTTGA560.14198782961460446No Hit
GGACTACAAGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA560.14198782961460446No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTCGCTATTGTGTGTTC550.1394523326572008No Hit
GGACTACAAGGGTATCTAATCGTTTTCGATATCCTAACCTTCGTTCTTGA540.13691683569979715No Hit
GGACTACGGGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA520.13184584178498987No Hit
GGACTACCAGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA520.13184584178498987No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTCGCTATTGTGTGTTC520.13184584178498987No Hit
GGACTACGAGGGTTTCTAATCCCAGTTTGGGTCTTCGCTATTGTGTGTTC510.12931034482758622No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTCGCTATTGTGTGTTC510.12931034482758622No Hit
GGACTACGCGGGTTTCTAATCGTTTTCGATATCCTAACCTTCGTTCTTGA510.12931034482758622No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTCGCTATTGTGTGTTC500.12677484787018256No Hit
GGACTACTAGGGTTTCTAATCATCTTCGAACCTCCGGCTTTTGTTCTTGA500.12677484787018256No Hit
GGACTACGAGGGTTTCTAATCGTTTTCGATATCCTAACCTTCGTTCTTGA500.12677484787018256No Hit
GGACTACAGGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA500.12677484787018256No Hit
GGACTACCCGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA460.11663286004056794No Hit
GGACTACTCGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA460.11663286004056794No Hit
GGACTACGAGGGTATCTAATCGTTTTCGATATCCTAACCTTCGTTCTTGA460.11663286004056794No Hit
GGACTACCAGGGTATCTAATCGTTTTCGATATCCTAACCTTCGTTCTTGA440.11156186612576065No Hit
GGACTACCGGGGTTTCTAATCGTTTTCGATATCCTAACCTTCGTTCTTGA440.11156186612576065No Hit
GGACTACCGGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA430.10902636916835699No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA420.10649087221095335No Hit
GGACTACGGGGGTTTCTAATCGTTTTCGATATCCTAACCTTCGTTCTTGA400.10141987829614604No Hit
GGACTACTGGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA400.10141987829614604No Hit
GGACTACCCGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA400.10141987829614604No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)