FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55710
Sequences flagged as poor quality0
Sequence length239
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG49648.910429007359541No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG47868.590917250044876No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG40007.1800394902171965No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG36706.587686232274277No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC23074.141087775982768No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC21413.8431161371387543No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC19053.4194938072159395No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC17173.0820319511757317No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC12032.1593968766828215No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC11692.0983665410159755No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC9321.6729492012206069No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC9111.6352539938969666No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT8891.5957637767007717No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT8371.5024232633279482No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT8141.4611380362591995No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT7711.3839526117393646No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT6041.0841859630227966No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT5991.0752109136600252No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT5961.0698258840423622No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT5601.0052055286304076No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG4810.8633997486986178No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG3500.6282534553940047No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT3400.6103033566684617No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG3330.5977382875605816No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC2920.5241428827858554No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT2790.5008077544426494No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC2660.4774726260994435No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG2600.4667025668641177No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT2410.432597379285586No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT2180.39131215221683724No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC2140.38413211272662No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC1800.32310177705977383No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGGGCTAGCGTTGTTCGGAATTAC1770.3177167474421109No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG1530.27463651050080773No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGGGCTAGCGTTGTTCGGAATTAC1450.2602764315203734No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG1440.25848142164781907No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT1410.25309639203015616No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGC1150.20642613534374438No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG1120.20104110572608147No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT1050.1884760366182014No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGC1040.1866810267456471No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGGGCTAGCGTTGTTCGGAATTAC1020.18309100700053849No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG1020.18309100700053849No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT1020.18309100700053849No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGC990.1777059773828756No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCACTAGAAGCCGGCG970.17411595763776702No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCACTAGAAGCCGGCG950.1705259378926584No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCACTAGAAGCCGGCG850.15257583916711542No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT850.15257583916711542No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCACTAGAAGCCGGCG840.1507808292945611No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC840.1507808292945611No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT810.14539579967689822No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC800.14360078980434393No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGGGCTAGCGTTGTTCGGAATTAC780.14001077005923532No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT780.14001077005923532No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGC730.13103572069646385No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT710.12744570095135524No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC710.12744570095135524No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGC700.12565069107880095No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGC680.12206067133369233No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGT660.11847065158858373No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGC660.11847065158858373No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCCATAGAAGCCGGCG630.11308562197092084No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC600.10770059235325795No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCA600.10770059235325795No Hit
GTGTCAGCAGCCGCGGTAACACGATTACCCCAAGTCAATAGAAGCCGGCG590.10590558248070364No Hit
GTGCCAGCCGCCGCGGTAACACGATTCACCCAAGTCAATAGAAGCCGGCG580.10411057260814933No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGATTA570.10231556273559504Illumina Single End PCR Primer 1 (96% over 31bp)
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCCATAGAAGCCGGCG570.10231556273559504No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC560.10052055286304074No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)