FastQCFastQC Report
Tue 7 May 2024
EGAF00004969496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27721
Total Bases6.6 Mbp
Sequences flagged as poor quality0
Sequence length239
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT330911.936798816781502No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT26429.530680711374048No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT17596.345369936149489No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT14275.147721943652826No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC12614.548897947404495No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG11884.285559683994085No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC10153.6614840734461236No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG9123.289924605894448No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT9063.268280365066195No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT6862.4746582013635874No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG6592.3772591176364486No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC6592.3772591176364486No Hit
GTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT5872.1175282276974134No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC5672.0453807582699035No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT4851.7495761336171134No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG4841.7459687601457379No Hit
GTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT4521.6305328090617224No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT3931.4176977742505683No Hit
GTGCCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT2720.9812055842141337No Hit
GTGCCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT2650.9559539699145053No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT2430.8765917535442443No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT2410.8693770066014935No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGT1760.6348977309620865No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGT1630.5880018758342052No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT1470.5302839002921973No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC1440.5194617798780707No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT1400.5050322859925688No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGT1370.49421016557844233No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC1080.3895963349085531No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGT970.3499152267234227No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGT970.3499152267234227No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC970.3499152267234227No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCACTAGCGTATATTAAAGTTGT930.3354857328379207No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGT830.2994119981241658No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC820.2958046246527903No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCACTAGCGTATATTAAAGTTGT800.2885898777100393No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG750.2705530103531618No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG730.2633382634104109No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC670.2416940225821579No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC600.2164424082825295No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTCTATTAAAGTTGT600.2164424082825295No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGT590.21283503481115398No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC560.2020129143970275No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTCTATTAAAGTTGT550.19840554092565205No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCACTAGCGTATATTAAAGTTGT540.19479816745427656No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTCTATTAAAGTTGT530.19119079398290104No Hit
GTGTCAGCAGCCGCGGTAATTCCCGCTCCAATAGCGTATATTAAAGTTGT530.19119079398290104No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGT520.18758342051152554No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTCTATTAAAGTTGT510.18397604704015008No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC480.17315392662602358No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG470.16954655315464812No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG450.1623318062118971No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC430.15511705926914615No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC430.15511705926914615No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC420.15150968579777066No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC410.14790231232639514No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC410.14790231232639514No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC370.13347281844089318No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC340.12265069802676672No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTCAC340.12265069802676672No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC310.11182857761264024No Hit
GTGTCAGCCGCCGCGGTAATTCCCGCTCCAATAGCGTATATTAAAGTTGT310.11182857761264024No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC300.10822120414126475No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCACTAGCGTATATTAAAGTTGT290.10461383066988925No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC290.10461383066988925No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTCAC290.10461383066988925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)