FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74926
Sequences flagged as poor quality0
Sequence length239
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA34034.541814590395857No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA24553.276566211995836No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA21212.83079304914182No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA20382.720017083522409No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA19002.5358353575527857No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA18242.434401943250674No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA16962.2635667191629074No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA16542.2075114112591088No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA16462.1968342097536233No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA16382.1861570082481383No Hit
GGACTACGCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA15942.1274323999679683No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA15632.0860582441342124No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA14711.96327042682113No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA14601.9485892747510878No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA14551.9419160238101596No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA13781.839147959319862No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA13391.7870966019806207No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA12691.6936710888076234No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA12621.6843285374903236No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA12071.6109227771401118No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA11881.5855644235645838No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11461.5295091156607854No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA11121.4841310092624724No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA11021.4707845073806156No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA10901.4547687051223874No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8591.1464645116514962No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8461.1291140592050823No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7070.9435976830472733No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6950.9275818807890452No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6710.895550276272589No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6280.8381603181806049No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6030.804794063475963No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6010.8021247630995916No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5770.7700931585831353No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5720.7634199076422069No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5600.7474041053839788No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5560.7420655046312362No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5560.7420655046312362No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5360.7153725008675226No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5290.7060299495502229No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5170.6900141472919947No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5140.6860101967274377No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5060.6753329952219523No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4790.6392974401409391No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4570.6099351360008541No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4530.6045965352481115No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4490.5992579344953688No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4460.5952539839308117No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4410.5885807329898833No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4310.5752342311080266No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4040.5391986760270133No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4040.5391986760270133No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4010.5351947254624563No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4000.5338600752742706No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3930.5245175239569709No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3930.5245175239569709No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3920.5231828737687851No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3870.5165096228278568No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3800.5071670715105571No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3640.48581266849958626No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3640.48581266849958626No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3610.4818087179350292No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3500.4671275658649868No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3430.45778501454768705No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3310.4417692122894589No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3280.43776526172490193No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3240.4324266609721592No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3240.4324266609721592No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3070.4097376077730027No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA2940.39238715532658885No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA2740.3656941515628754No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA2690.35902090062194697No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA1950.2602567866962069No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1940.25892213650802126No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1720.22955983236793634No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA1480.1975282278514801No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1450.19352427728692312No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA1390.18551637615780905No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA1340.17884312521688064No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA1310.17483917465232363No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1260.16816592371139524No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1230.16416197314683822No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1190.1588233723940955No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1130.15081547126498143No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1110.1481461708886101No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1110.1481461708886101No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1100.1468115207004244No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1070.1428075701358674No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1060.1414729199476817No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA1050.14013826975949603No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1040.13880361957131035No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1030.1374689693831247No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA990.13213036863038197No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA980.13079571844219628No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA940.1254571176894536No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA910.12145316712489657No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA910.12145316712489657No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA900.12011851693671088No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA890.1187838667485252No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA890.1187838667485252No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA890.1187838667485252No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA870.11611456637215387No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA860.11477991618396817No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA850.11344526599578252No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA840.11211061580759683No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA840.11211061580759683No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA840.11211061580759683No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA840.11211061580759683No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA840.11211061580759683No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA830.11077596561941115No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA830.11077596561941115No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA810.1081066652430398No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA790.10543736486666845No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA790.10543736486666845No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA790.10543736486666845No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA780.10410271467848277No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA770.10276806449029709No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA760.10143341430211142No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA760.10143341430211142No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA750.10009876411392574No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA750.10009876411392574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)