FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004969514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350023
Sequences flagged as poor quality0
Sequence length239
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGAATTAT296538.47172900066567No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGAATTAT241266.8926899089488405No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT231096.602137573816577No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT197305.636772440668185No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT192885.510495024612668No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGAATTAT176875.053096510800719No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT160534.586270045111322No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGAATTAT146224.177439768243802No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT140534.014879022235681No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT118803.3940626758812993No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT115403.29692620199244No Hit
GTGTCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT103302.9512346331526786No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT96882.7678181148095984No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT89752.5641172151544325No Hit
GTGTCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT86112.4601240489910663No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTTCAGGCGTTATCCGGATTTAT74512.1287172557231955No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT72322.066149938718313No Hit
GTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT63051.8013101996154537No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTTCAGGCGTTATCCGGATTTAT61651.761312828014159No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT53561.5301851592609628No Hit
GTGCCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT52271.4933304382854842No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTTCAGGCGTTATCCGGATTTAT45901.3113423974995928No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT44241.263916942600915No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTTCAGGCGTTATCCGGATTTAT37841.0810718152807102No Hit
GTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT28040.8010902140716467No Hit
GTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT22440.6411007276664676No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC17730.5065381417792545No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC17050.4871108470014827No Hit
GTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT16510.4716832893838405No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC15400.43997108761424253No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC14290.4082588858446445No Hit
GTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT13880.39654536987569383No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC10380.29655194087245695No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC10350.2956948543381435No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT9270.26483973910285896No Hit
GTGTCAGCAGCCGCGGTAATACCTAGGTCGCAAGCGTTATCCGGAATTAT9230.26369695705710766No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC8870.2534119186453462No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC8630.2465552263708385No Hit
GTGTCAGCCGCCGCGGTAATACCTAGGTCGCAAGCGTTATCCGGAATTAT7850.22427097647868854No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT7760.22169971687574816No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT7530.2151287201126783No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT6450.18427360487739378No Hit
GTGCCAGCAGCCGCGGTAATACCTAGGTCGCAAGCGTTATCCGGAATTAT6050.172845784419881No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT5510.15741822680223871No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTATCCGGAATTAT5230.14941875248197975No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATGAC4930.14084788713884516No Hit
GTGCCAGCCGCCGCGGTAATACCTAGGTCGCAAGCGTTATCCGGAATTAT4870.13913371407021824No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT4840.13827662753590478No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT4460.12742019810126762No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGAATTAT4170.11913502826957086No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTATCCGGAATTAT4010.11456390008656574No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATGAC3880.11084985843787408No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT3720.10627873025486896No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)