FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59895
Sequences flagged as poor quality0
Sequence length239
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA19023.1755572251440016No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA15972.666332748977377No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA14912.489356373653894No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA12412.0719592620419065No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA11551.9283746556473829No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA10691.784790049252859No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA10541.75974622255614No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA10191.7013106269304616No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA9971.6645796811086067No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA9471.5811002587862093No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA9281.5493780783036981No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA8771.4642290675348526No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA8761.462559479088405No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA8291.384088822105351No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA8141.3590449954086319No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA7791.3006093997829535No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA7741.2922614575507136No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA7651.2772351615326822No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7451.2438433926037231No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA6691.116954670673679No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA6631.1069371399949912No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA6601.1019283746556474No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA6081.015109775440354No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA6061.0117705985474579No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5890.9833875949578429No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA5660.9449870606895401No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA5620.9383087069037482No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5490.9166040570999248No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA5470.9132648802070289No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA5400.9015777610818932No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA5400.9015777610818932No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA5340.8915602304032056No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5200.8681859921529342No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA4920.8214375156523916No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA4920.8214375156523916No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA4860.811419984973704No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA4860.811419984973704No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA4820.8047416311879121No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA4780.7980632774021205No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA4620.7713498622589532No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA4400.7346189164370982No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA4190.6995575590616913No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA4040.674513732364972No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA3990.6661657901327324No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3940.6578178479004926No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3920.6544786710075966No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA3880.6478003172218049No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3840.641121963436013No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3500.5843559562567827No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA3470.5793471909174388No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA3460.577677602470991No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3400.5676600717923033No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3360.5609817180065114No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA3320.5543033642207196No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3280.5476250104349277No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3180.5309291259704483No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA2980.4975373570414892No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2850.4758327072376659No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2810.4691543534518741No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA2790.4658151765589782No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2760.46080641121963434No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA2730.45579764588029054No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA2720.45412805743384255No Hit
GGACTACGCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA2680.4474497036480508No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA2650.4424409383087069No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2640.4407713498622589No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA2470.4123883462726438No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA2380.39736205025461224No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA2320.38734451957592453No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2220.370648635111445No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2140.35729192753986144No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA2100.3506135737540696No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA2080.34727439686117373No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA2030.33892645462893395No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA2030.33892645462893395No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA1990.33224810084314216No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA1970.3289089239502463No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA1960.3272393355037983No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA1950.32556974705735037No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1860.3105434510393188No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1840.30720427414642293No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1820.303865097253527No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA1780.29718674346773516No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1760.2938475665748393No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA1710.2854996243425995No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA1680.2804908590032557No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1610.26880373987812No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA1580.2637949745387762No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA1540.25711662075298436No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA1500.25043826696719257No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA1490.2487686785207446No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1380.23040320560981717No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA1340.2237248518240254No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA1260.21036814425244177No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCA1250.2086985558059938No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA1210.20202020202020202No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA1200.20035061357375405No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCA1150.19200267134151433No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA1140.19033308289506637No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA1120.18699390600217045No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA1110.1853243175557225No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA1100.18365472910927455No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA1090.18198514066282662No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA1090.18198514066282662No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA1090.18198514066282662No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA1050.1753067868770348No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA1010.168628433091243No Hit
GGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA1010.168628433091243No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1000.16695884464479505No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA1000.16695884464479505No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA940.15694131396610736No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA920.15360213707321146No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA910.1519325486267635No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA860.14358460639452375No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA860.14358460639452375No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCA860.14358460639452375No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA840.14024542950162786No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCA810.135236664162284No Hit
GGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA780.13022789882294017No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA760.12688872193004425No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCA740.12354954503714835No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA740.12354954503714835No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA720.12021036814425246No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA720.12021036814425246No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCA710.11854077969780448No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA710.11854077969780448No Hit
GGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA700.11687119125135653No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA700.11687119125135653No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA690.11520160280490858No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCA680.11353201435846064No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCA680.11353201435846064No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA670.1118624259120127No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCA670.1118624259120127No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCA630.10518407212622088No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTA610.10184489523332499No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCA600.10017530678687703No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA600.10017530678687703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)