FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72552
Sequences flagged as poor quality0
Sequence length239
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA24903.4320211710221633No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA20182.781453302458926No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA19322.6629176314918954No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA18472.545760282280295No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA17462.4065497849818063No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA17372.3941448891829307No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA17332.3886316021612086No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA17012.3445253059874296No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA16982.3403903407211377No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA15642.155695225493439No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA15372.1184805380968132No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA14201.9572168927114344No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA13661.8827875179181826No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA13281.8304112912118204No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA13211.8207630389238065No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA13021.7945749255706251No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA13021.7945749255706251No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA12711.751846951152277No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA12471.718767229021943No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA12411.7104972984893594No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA11861.6346896019406771No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA11791.625041349652663No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA11341.5630168706582865No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA11341.5630168706582865No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA11271.5533686183702722No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA11211.5450986878376889No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA10961.5106406439519242No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA10541.4527511302238394No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA10521.4499944867129784No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA10331.4238063733597972No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA9971.374186790164296No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA9441.3011357371264747No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA9281.2790825890395854No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA8961.2349762928658066No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA8961.2349762928658066No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA8671.1950049619583196No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA8481.1688168486051385No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA8481.1688168486051385No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8141.1219539089204984No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA7741.0668210387032748No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA7711.062686073436983No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA7651.0544161429043997No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA7561.0420112471055243No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA7551.0406329253500939No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA6810.9386371154482303No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA6480.8931524975190208No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA5860.8076965486823243No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4890.6739993384055575No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA4780.6588377990958209No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4390.605083250634028No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4130.5692468849928327No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3910.5389238063733598No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3750.5168706582864704No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3650.5030874407321645No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3500.4824126144007057No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3480.47965597088984446No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3380.4658727533355386No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3010.4148748483846069No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2970.4093615613628846No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2900.39971330907487046No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2890.39833498731943984No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2780.3831734480097034No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2710.3735251957216893No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2540.35009372587936927No Hit
GGACTACTAGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA2500.34458043885764694No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2390.3294188995479104No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2300.31701400374903516No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2290.3156356819936046No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2270.3128790384827434No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2150.29633917741757637No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2100.2894475686404234No Hit
GGACTACAAGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA2010.27704267284154815No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1990.27428602933068696No Hit
GGACTACCAGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1880.2591244900209505No Hit
GGACTACAGGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1840.2536112029992281No Hit
GGACTACTGGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1780.2453412724666446No Hit
GGACTACTAGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1770.24396295071121402No Hit
GGACTACGAGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1740.23982798544492223No Hit
GGACTACTCGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1710.23569302017863047No Hit
GGACTACGGGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1660.22880141140147756No Hit
GGACTACTCGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1550.21363987209174112No Hit
GGACTACGCGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1540.2122615503363105No Hit
GGACTACCGGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1540.2122615503363105No Hit
GGACTACACGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1530.2108832285808799No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1460.20123497629286582No Hit
GGACTACGAGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1350.18607343698312934No Hit
GGACTACAAGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1300.1791818282059764No Hit
GGACTACGGGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1150.15850700187451758No Hit
GGACTACTGGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1130.15575035836365642No Hit
GGACTACAGGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1120.15437203660822582No Hit
GGACTACGCGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1050.1447237843202117No Hit
GGACTACCAGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1050.1447237843202117No Hit
GGACTACACGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1040.14334546256478112No Hit
GGACTACCCGGGTTTCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA1010.13921049729848936No Hit
GGACTACCGGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA960.13231888852133644No Hit
GGACTACCCGGGTATCTAATCGTTTTCGATACCCTAACCTTCGTTCTTGA780.10750909692358583No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)