FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9766
Sequences flagged as poor quality0
Sequence length239
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC9279.492115502764694No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC6366.512389924226909No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT6286.430473069834118No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT5005.119803399549458No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC4744.853573622772886No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC3984.075363506041367No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC3763.850092156461192No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT3743.8296129428629944No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT2632.693016588163015No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG2602.662297767765718No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC2582.6418185541675197No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC1962.006962932623387No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG1741.7816915830432112No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC1401.4335449518738481No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG1281.310669670284661No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATCAT1191.2185132090927708No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATCAT1141.1673151750972763No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG1111.1365963546999795No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC950.9727626459143969No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT910.9318042187180013No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT880.9010853983207044No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC780.7986893303297153No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT780.7986893303297153No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT740.7577309031333196No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT730.7474912963342207No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATCAT680.6962932623387261No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGAATTAC650.6655744419414295No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC650.6655744419414295No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAT650.6655744419414295No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT620.6348556215441327No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC590.604136801146836No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGAATTAC530.5426991603522424No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATCAT520.5324595535531436No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC480.4915011263567479No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT470.481261519557649No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT460.4710219127585501No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT440.4505426991603522No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT440.4505426991603522No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT430.4403030923612533No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAT420.43006348556215446No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT420.43006348556215446No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT420.43006348556215446No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTGTCCGGAATTAT380.38910505836575876No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC370.3788654515666598No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC340.34814663116936306No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT320.32766741757116524No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT320.32766741757116524No Hit
GTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT310.31742781077206633No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC310.31742781077206633No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAT300.3071882039729675No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGAATTAC300.3071882039729675No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT290.2969485971738685No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT280.2867089903747696No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGAATTAC280.2867089903747696No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT270.2764693835756707No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTGTCCGGAATTAT270.2764693835756707No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT260.2662297767765718No Hit
GTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT250.25599016997747287No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC240.24575056317837396No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT240.24575056317837396No Hit
GTGCCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT240.24575056317837396No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAT220.2252713495801761No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT220.2252713495801761No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC210.21503174278107723No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC210.21503174278107723No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG200.20479213598197826No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT190.19455252918287938No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT190.19455252918287938No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGGGCTAGCGTTGTTCGGAATTAC190.19455252918287938No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTGTCCGGAATTAT190.19455252918287938No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG180.18431292238378047No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT180.18431292238378047No Hit
GTGCCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT180.18431292238378047No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC180.18431292238378047No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTGTCCGGAATTAT170.17407331558468153No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAAC170.17407331558468153No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT160.16383370878558262No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT160.16383370878558262No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC160.16383370878558262No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT150.15359410198648374No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC150.15359410198648374No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTAT150.15359410198648374No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC150.15359410198648374No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT140.1433544951873848No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT140.1433544951873848No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT130.1331148883882859No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT130.1331148883882859No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT130.1331148883882859No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC130.1331148883882859No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT120.12287528158918698No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT110.11263567479008806No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC100.10239606799098913No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTAT100.10239606799098913No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGAATTAT100.10239606799098913No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCGAGCGTTAATCGGAATTAC100.10239606799098913No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)