FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12006
Sequences flagged as poor quality0
Sequence length239
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC5514.5893719806763285No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT5034.189571880726303No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT5024.181242711977345No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC4733.939696818257538No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC4723.931367649508579No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT4583.8147592870231546No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC3763.1317674496085286No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT3693.073463268365817No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC3562.965184074629352No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC3072.557054805930368No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC2902.4154589371980677No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC2482.0656338497417956No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC2191.824087956021989No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT1991.657504581042812No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG1951.6241879060469766No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT1831.5242378810594703No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC1671.3909711810761287No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC1591.3243378310844578No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC1511.2577044810927869No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT1491.2410461435948694No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT1431.191071131101116No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG1431.191071131101116No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG1411.1744127936031985No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG1371.141096118607363No Hit
GTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT1341.1161086123604864No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT1341.1161086123604864No Hit
GTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT1180.9828419123771448No Hit
GTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT1160.966183574879227No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT1120.9328668998833917No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT1040.8662335498917209No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT1020.8495752123938031No Hit
GTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT1010.8412460436448441No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT960.7996001999000499No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT840.6996501749125438No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT820.682991837414626No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT800.6663334999167083No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT780.6496751624187905No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATCAT730.6080293186739963No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATCAT700.5830418124271198No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT630.5247376311844079No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT620.5164084624354489No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATCAT600.49975012493753124No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT580.4830917874396135No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT570.4747626186906547No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT570.4747626186906547No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT560.46643344994169583No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC550.45810428119273694No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT540.4497751124437781No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC530.44144594369481926No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGAATTAT500.41645843744794275No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGAATTAT480.39980009995002497No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAT460.38314176245210724No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC410.341495918707313No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC400.33316674995835416No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGAATTAT400.33316674995835416No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC390.32483758120939527No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC390.32483758120939527No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGAATTAC390.32483758120939527No Hit
GTGTCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT380.31650841246043643No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC380.31650841246043643No Hit
GTGCCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT370.3081792437114776No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC350.2915209062135599No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC340.283191737464601No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAT340.283191737464601No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC330.2748625687156422No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGAATTAC310.25820423121772446No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC300.24987506246876562No Hit
GTGTCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT300.24987506246876562No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC290.24154589371980675No Hit
GTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT290.24154589371980675No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAT280.23321672497084792No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT280.23321672497084792No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT270.22488755622188905No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAT250.20822921872397138No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATCAT250.20822921872397138No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGAATTAT240.19990004997501248No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT230.19157088122605362No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT230.19157088122605362No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT220.18324171247709478No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTGTCCGGAATTAT220.18324171247709478No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTGTCCGGAATTAT220.18324171247709478No Hit
GTGTCAGCAGCCGCGGTAGTCCGTACAAGAGTGAAGATCGGAAGAGCGTC210.17491254372813594No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAAC210.17491254372813594No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGAATTAC210.17491254372813594No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC200.16658337497917708No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTGTCCGGAATTAT200.16658337497917708No Hit
GTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT180.14992503748125938No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC180.14992503748125938No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGAATTAC180.14992503748125938No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAAC170.1415958687323005No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTGTCCGGAATTAT170.1415958687323005No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT170.1415958687323005No Hit
GTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT170.1415958687323005No Hit
GTGCCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT160.13326669998334167No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAAC150.12493753123438281No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT150.12493753123438281No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT150.12493753123438281No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT150.12493753123438281No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT140.11660836248542396No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG130.1082791937364651No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG130.1082791937364651No Hit
GTGCCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT130.1082791937364651No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG130.1082791937364651No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC130.1082791937364651No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)