FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64024
Sequences flagged as poor quality0
Sequence length239
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA21123.2987629638885423No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA17322.7052355366737473No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA16072.5099962514057226No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA13852.1632512807697113No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA12581.9648881669373983No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA12361.9305260527302261No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA12281.9180307384730728No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA11771.8383731100837186No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA11011.7196676246407596No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA10701.6712482818942898No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA9861.5400474821941772No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA9821.5337998250656004No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA9651.5072472822691492No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA9371.4635136823691115No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA9351.4603898538048232No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA9261.4463326252655255No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA9121.4244658253155067No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA8841.3807322254154692No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8821.377608396851181No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA8321.299512682743971No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA7651.1948644258403098No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA7541.1776833687367236No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA7191.123016368861677No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA7051.1011495689116582No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA7011.0949019117830814No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6941.083968511808072No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA6521.018368111958016No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA6491.013682369111583No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA6360.9933774834437087No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA6210.9699487692115457No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA6190.9668249406472573No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6160.9621391978008247No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6100.9527677121079594No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA5980.9340247407222292No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA5860.9152817693364987No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA5650.8824815694114706No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA5640.8809196551293265No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5590.8731100837186055No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA5550.8668624265900288No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA5440.8496813694864426No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5370.8387479695114333No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA5280.8246907409721356No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA5090.7950143696113958No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA4990.7793952267899538No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4970.7762713982256654No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA4960.7747094839435211No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA4870.7606522554042234No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA4720.7372235411720605No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA4690.7325377983256278No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4630.7231663126327628No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA4500.7028614269648882No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA4500.7028614269648882No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA4470.6981756841184555No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA4430.6919280269898788No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA4360.6809946270148695No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA4300.6716231413220043No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA4110.6419467699612645No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA4080.6372610271148319No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA4080.6372610271148319No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3940.6153942271648132No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA3880.606022741471948No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA3820.5966512557790828No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA3730.582594027239785No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA3680.5747844558290641No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3570.557603398725478No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA3490.5451080844683244No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA3460.5404223416218917No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA3450.5388604273397476No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3440.5372985130576033No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA3420.534174684493315No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA3340.5216793702361614No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA3310.5169936273897289No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3240.5060602274147195No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3190.49825065600399854No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA3180.49668874172185434No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA3160.49356491315756595No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA3030.4732600274896913No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3000.4685742846432588No Hit
GGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA2900.45295514182181684No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA2870.44826939897538426No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA2690.4201549418967887No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA2630.4107834562039235No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA2600.40609771335749095No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA2530.39516431338248154No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA2420.3779832562788954No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA2330.36392602773959765No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA2270.3545545420467325No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA2250.3514307134824441No Hit
GGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA2100.3280019992502812No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA2080.32487817068599273No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA2020.31550668499312756No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA2020.31550668499312756No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA1940.303011370735974No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA1930.3014494564538298No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA1920.2998875421716856No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA1890.29520179932525303No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA1880.29363988504310884No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA1830.2858303136323878No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA1570.24522054229663875No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA1470.22960139947519678No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA1460.2280394851930526No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA1440.22491565662876423No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA1300.20304885667874548No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA1230.19211545670373611No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA1190.1858677995751593No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA1190.1858677995751593No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA1160.18118205672872673No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA1090.17024865675371736No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA1030.1608771710608522No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA1010.15775334249656378No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA960.1499437710858428No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA900.14057228539297764No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA830.12963888541796825No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA740.1155816568786705No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA710.1108959140322379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)