FastQCFastQC Report
Tue 7 May 2024
EGAF00004969558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14758
Total Bases3.5 Mbp
Sequences flagged as poor quality0
Sequence length239
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC185012.535573926006233No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC183312.420382165605096No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC152810.353706464290555No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC148210.042011112616885No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC6514.411166824773004No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC6144.1604553462528795No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC5073.4354248543163033No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC5053.4218728825044042No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC2011.3619731670958124No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC1891.2806613362244206No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC1881.2738853503184715No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG1861.2603333785065727No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG1741.1790215476351809No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC1711.158693589917333No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG1611.09093373085784No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG1450.9825179563626508No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT740.5014229570402494No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC670.4539910556986042No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT640.43366309798075614No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT620.4201111261688576No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT530.35912725301531373No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT510.34557528120341513No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC500.3387992952974658No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT460.3116953516736685No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC450.3049193657677192No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC450.3049193657677192No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC420.28459140804987126No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT420.28459140804987126No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT410.27781542214392196No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGATTAGAAA410.27781542214392196Illumina Single End PCR Primer 1 (96% over 25bp)
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC400.2710394362379726No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT390.2642634503320233No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT380.25748746442607395No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC370.2507114785201247No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGATTA360.24393549261417535Illumina Single End PCR Primer 1 (96% over 31bp)
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC360.24393549261417535No Hit
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGATTAGAAA360.24393549261417535Illumina Single End PCR Primer 1 (96% over 25bp)
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT360.24393549261417535No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGATTAGAAA360.24393549261417535Illumina Single End PCR Primer 1 (96% over 25bp)
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC350.23715950670822605No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTCAC330.22360753489632743No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC320.21683154899037807No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC320.21683154899037807No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC320.21683154899037807No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTCAC320.21683154899037807No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT320.21683154899037807No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGATTA290.19650359127253014Illumina Single End PCR Primer 1 (96% over 31bp)
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGATTAGAAA290.19650359127253014Illumina Single End PCR Primer 1 (96% over 25bp)
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC280.18972760536658084No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTCAC270.18295161946063151No Hit
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGATTA260.17617563355468221Illumina Single End PCR Primer 1 (96% over 31bp)
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGATTAGATA250.1693996476487329Illumina Single End PCR Primer 1 (96% over 25bp)
GTCCCAGCCTGGAGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA250.1693996476487329Illumina Single End PCR Primer 1 (100% over 34bp)
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG230.15584767583683426No Hit
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGATTAGATA200.1355197181189863Illumina Single End PCR Primer 1 (96% over 25bp)
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGATTAGATA200.1355197181189863Illumina Single End PCR Primer 1 (96% over 25bp)
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCTAGTGTTGCTCGGAATTAC200.1355197181189863No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTCAC190.12874373221303698No Hit
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGATTA180.12196774630708768Illumina Single End PCR Primer 1 (96% over 31bp)
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC170.11519176040113836No Hit
GTGTCAGAAACCCCGGTAGTCCCAGCCTGGAGTAAGATCGGAAGAGCGTC170.11519176040113836No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATCAC150.10163978858923972No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATCAC150.10163978858923972No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC150.10163978858923972No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC150.10163978858923972No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)