FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14758
Sequences flagged as poor quality0
Sequence length239
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA5663.8352080227673127No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA4953.3541130234449117No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA4352.9475538690879524No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA3912.6494104892261823No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA3732.527442742919095No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA3632.459682883859602No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA3532.3919230248001084No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA3302.2360753489632743No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA3242.195419433527578No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA3122.1141076026561865No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA3102.100555630844288No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA3092.093779644938339No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA3082.0870036590323893No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2982.019243799972896No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2861.9379319691015042No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2841.9243799972896058No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2761.870172110042011No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2731.849844152324163No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2691.822740208700366No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2591.7549803496408727No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2591.7549803496408727No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2481.6804445046754304No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2371.6059086597099876No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2261.5313728147445453No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1761.1925735194470795No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1511.0231738717983467No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1360.9215340832091069No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1210.8198942946198672No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1140.772462393278222No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1130.7656864073722727No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1090.7385824637484755No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1070.7250304919365768No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1060.7182545060306275No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1000.6775985905949315No Hit
GGACTACGCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA930.6301666892532863No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA920.623390703347337No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA870.5895107738175904No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA820.5556308442878439No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA810.5488548583818945No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA800.5420788724759452No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA790.535302886569996No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA760.5149749288521479No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA720.4878709852283507No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA710.48109499932240146No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA700.4743190134164521No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA700.4743190134164521No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA690.46754302751050275No Hit
GGACTACGGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC590.39978316845100964No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA560.3794552107331617No Hit
GGACTACGGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC530.35912725301531373No Hit
GGACTACGCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC450.3049193657677192No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC350.23715950670822605No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC350.23715950670822605No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC330.22360753489632743No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC330.22360753489632743No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC320.21683154899037807No Hit
GGACTACGAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC320.21683154899037807No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC310.2100555630844288No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC290.19650359127253014No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC290.19650359127253014No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC290.19650359127253014No Hit
GGACTACGCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC280.18972760536658084No Hit
GGACTACGAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC260.17617563355468221No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA260.17617563355468221No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC250.1693996476487329No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC250.1693996476487329No Hit
GGACCACTATGCCGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC240.16262366174278356Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC220.14907168993088493No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC210.14229570402493563No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA210.14229570402493563No Hit
GGACTACTCGGGTTTCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA210.14229570402493563No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC210.14229570402493563No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA190.12874373221303698No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC190.12874373221303698No Hit
GGACTACGGGGGTTTCTAATCCCTACACGACGCTCTTCCGATCTTACCGC190.12874373221303698Illumina Single End PCR Primer 1 (96% over 26bp)
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC190.12874373221303698No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC180.12196774630708768No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA180.12196774630708768No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC180.12196774630708768No Hit
GGACTACCCGGGTTTCTAATCCCTACACGACGCTCTTCCGATCTTACCGC180.12196774630708768Illumina Single End PCR Primer 1 (96% over 26bp)
GGACTACCGGGGTTTCTGACACCACTATGCCGAAAGATCGGAAGAGCACA170.11519176040113836No Hit
GGACTACTGGGGTTTCTAATCGTCTTCGAACCTCCGACTTTCGTTCTTGA170.11519176040113836No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA160.10841577449518904No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA160.10841577449518904No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA160.10841577449518904No Hit
GGACTACCAGGGTTTCTAATCCCTACACGACGCTCTTCCGATCTTACCGC150.10163978858923972Illumina Single End PCR Primer 1 (96% over 26bp)
GGACTACGGGGGTATCTAATCTTTCCCTACACGACGCTCTTCCGATCTTA150.10163978858923972Illumina Single End PCR Primer 1 (96% over 31bp)
GGACTACTGGGGTTTCTAATCCCTACACGACGCTCTTCCGATCTTACCGC150.10163978858923972Illumina Single End PCR Primer 1 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)