FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837
Sequences flagged as poor quality0
Sequence length239
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC16219.35483870967742No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC12414.814814814814813No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC11013.142174432497015No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC708.363201911589007No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC172.031063321385902No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC172.031063321385902No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC151.7921146953405016No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC141.6726403823178015No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC141.6726403823178015No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC121.4336917562724014No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC111.3142174432497014No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC111.3142174432497014No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC111.3142174432497014No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT80.955794504181601No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT70.8363201911589008No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC70.8363201911589008No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC70.8363201911589008No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC70.8363201911589008No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC70.8363201911589008No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC70.8363201911589008No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC70.8363201911589008No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAGTTAC60.7168458781362007No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC60.7168458781362007No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT50.5973715651135006No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC50.5973715651135006No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC50.5973715651135006No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT50.5973715651135006No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTAT40.4778972520908005No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC40.4778972520908005No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT40.4778972520908005No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC40.4778972520908005No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC40.4778972520908005No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC30.35842293906810035No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC30.35842293906810035No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAGTTAC30.35842293906810035No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC30.35842293906810035No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC30.35842293906810035No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC30.35842293906810035No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTCAC30.35842293906810035No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAGGCGTTAATCGGAATTAC20.23894862604540024No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTGTCCGGATTTAT20.23894862604540024No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC20.23894862604540024No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC20.23894862604540024No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGACTAGCGTTGTTCGGAATCAC20.23894862604540024No Hit
GTGTCAGCCGCCGCGGTAAGACGTAGGGTGCAAGCGTTGTCCGGAATTAC20.23894862604540024No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATCAC20.23894862604540024No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC20.23894862604540024No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT20.23894862604540024No Hit
GTGCCAGCCACCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC20.23894862604540024No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT20.23894862604540024No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC20.23894862604540024No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATCAC20.23894862604540024No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGCTAATCGGACTTAC10.11947431302270012No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTACCCGGAATCAC10.11947431302270012No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAACTAC10.11947431302270012No Hit
GTGCCAACCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC10.11947431302270012No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGACTAGCGTTGTTCGGAATCAC10.11947431302270012No Hit
GTGCCAGCAGCCGCAGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGTCAGCCGCCGCGATAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC10.11947431302270012No Hit
GTGCCAGCAGCCACGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGTCAGCAGCCGCGGTAAGATCATTAGAAACCCTGGTAGTCCGCACATC10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATCAC10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGATTTAC10.11947431302270012No Hit
GTGCCAGCCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTA10.11947431302270012No Hit
GCGTCAGCAGCCGCGGTCAGACGTAGGGTGCAAGCGTTGTCCGGAATTAC10.11947431302270012No Hit
GTGTCAGCAGCCGCGGCAATACCGAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGCCAGCAGCCGCGGTACTACAGAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT10.11947431302270012No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC10.11947431302270012No Hit
GTGTCAGCCGCCGCGATAATACAGAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTGATACGTTGGGTGCGAGCGTTAATCGGAATTAT10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGATGCAAGCGTTAATCGGACTTAC10.11947431302270012No Hit
GTGCCAGCAGCCGCGGTATTACCGAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGCCAGAGGCCGGGGAAATAGAGGGGGGGCAGGCGTTAATGGGAATTAC10.11947431302270012No Hit
GTGTCAGCAGCCGCGGTCATACAGAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTAT10.11947431302270012No Hit
GTTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTA10.11947431302270012No Hit
GAGTCATGCGCCCCTGAAACACTATTAACCGAATTCAAGAGAAAGCGGCG10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTCATACAGAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGCCAGTAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGTCAGCCGCCGTGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGCCAGCAGCCGCGGTATCATTATTAGAAACCCCAGTAGTCCGCACATC10.11947431302270012No Hit
GTGTCAGCAGCCGCGGTAAGATCATTAGAAACCCGTGTAGTCCGCACATC10.11947431302270012No Hit
GTGCCAGCAGCCGCGATAATACAGAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGTCAGCAGCCGCGGTAAGACGTAGGGTGCAAGCGTTGTCCGGAATTAC10.11947431302270012No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTCT10.11947431302270012No Hit
GTGCCAGCCGCCGCGGTAGCCCGCACATCGAGCAAGATCGGAAGAGCGTC10.11947431302270012No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCG10.11947431302270012No Hit
GCGTCAGCAGCCGCGGTCATACCGAGGGTGCAAGCGTTAATCGGCCTTCC10.11947431302270012No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATCAC10.11947431302270012No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTAT10.11947431302270012No Hit
GTGCCAGCCGCCGCGGTAAGATCATTAGAAACCCTAGTAGTCCGCACATC10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAGTTAC10.11947431302270012No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAT10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTAT10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAACACAGAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGCCAGCAGCCGCGGTAAGATCATTAGATACCCGCGTAGTCCGCACATC10.11947431302270012No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT10.11947431302270012No Hit
GTGCCAGCCGCCGCAGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAT10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGGGCTAGCGTTGTTCGGAATTAC10.11947431302270012No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGCGCTAGCGTTGTTCGGAATTAC10.11947431302270012No Hit
GTGTCAGCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACT10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTGATCGGAATTAC10.11947431302270012No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATGAC10.11947431302270012No Hit
GTGCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACT10.11947431302270012No Hit
GGGGCAGCAGCCGCGGTAATACGTAGGGTGCGGGCGTTAATCGGAGTTAT10.11947431302270012No Hit
GTGTCAGCAGCCGCGGTAAGGAAAGAGTGTAGATCTCGGTGGTCGCCGTA10.11947431302270012Illumina Single End PCR Primer 1 (97% over 35bp)
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCAGAATTAC10.11947431302270012No Hit
GTGCCAGCAGCCGCTGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
TTGTCAGCAGCCGCGGTATTACTCAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGGGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAAGATCATTAGAAACCCCCGTAGTCCGCACATC10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAAATAC10.11947431302270012No Hit
GTGCCACAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACT10.11947431302270012No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTCCGAGCGTTGTCCGGAATTAT10.11947431302270012No Hit
GTGCCAGCAGCCGCGGTAAGATCATTAGATACCCCCGTAGTCCGCACATC10.11947431302270012No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC10.11947431302270012No Hit
GTGTCAGCCGCCGGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACT10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC10.11947431302270012No Hit
GTGTCAGCCGCCGCTGTAATCCGTAGGGTGCGAGCGTTGTCCGGCATTGC10.11947431302270012No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCATGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGTCAGCAGCCGCGGTAAGATCATTAGAAACCCCGGTAGTCCGCACATC10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGGC10.11947431302270012No Hit
GTGTCAGCAACCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGTGCAAGAGTTAATCGGAATTAC10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAAGATCATTAGAAACCCCGGTAGTCCGCACATC10.11947431302270012No Hit
GTGCCAGCCGCCGCGGTAATACGCAGGGGGCTAGCGTTGCTCGGAATGAC10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACACCGGCG10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAGGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAATCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAAGACGTAGGATGCGAGCGTTGTCCGGATTTAT10.11947431302270012No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAGTTAC10.11947431302270012No Hit
GTGCCAGCAGTCGCGGTAATACGGAGGGGGCTAGCGTTGTTCGGAATTAC10.11947431302270012No Hit
GTGTCAGCAGCCGCAGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGCCGGTCTCTTCGGCTCTTCCATTTCCTTGGGCGTATACCACGGTGGT10.11947431302270012No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGCGCTAGCGTTGTTCGGAATTAC10.11947431302270012No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAACTAC10.11947431302270012No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTCAC10.11947431302270012No Hit
GTGCCAGCCGCCGTGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC10.11947431302270012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)