FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1660
Sequences flagged as poor quality0
Sequence length239
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC23614.216867469879519No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC23113.915662650602409No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC23113.915662650602409No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC19711.867469879518072No Hit
GTGTCAGCAGCCGCGGTAGTCCCGATTATGGCACAGATCGGAAGAGCGTC1649.879518072289157No Hit
GTGTCAGCCGCCGCGGTAGTCCCGATTATGGCACAGATCGGAAGAGCGTC603.614457831325301No Hit
GTGTCAGAAACCCCGGTAGTCCCGATTATGGCACAGATCGGAAGAGCGTC513.072289156626506No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC301.8072289156626504No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC271.6265060240963853No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC211.2650602409638554No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC171.0240963855421688No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC130.783132530120482No Hit
GTGTCAGCAGCCGCGGTAGTCCCGATTATGGCACAGATCGGACGAGCGTC100.6024096385542169No Hit
GTGTCAGCAGCCCCGGTAGTCCCGATTATGGCACAGATCGGAAGAGCGTC90.5421686746987951No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC90.5421686746987951No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC90.5421686746987951No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC80.48192771084337355No Hit
GTGTCAGCAGCCGCGGTAGTCCCGATTATGGCACAGATCGGCAGAGCGTC80.48192771084337355No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC70.42168674698795183No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC70.42168674698795183No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC60.3614457831325301No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC60.3614457831325301No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC60.3614457831325301No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC50.30120481927710846No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC50.30120481927710846No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC50.30120481927710846No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC50.30120481927710846No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC50.30120481927710846No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC40.24096385542168677No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC40.24096385542168677No Hit
GTGTCAGCAGCCGCGTAGTCCCGATTATGGCACAGATCGGAAGAGCGTCG40.24096385542168677No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC40.24096385542168677No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC40.24096385542168677No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC40.24096385542168677No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC30.18072289156626506No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC30.18072289156626506No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC30.18072289156626506No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT30.18072289156626506No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC30.18072289156626506No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC30.18072289156626506No Hit
GTGTCAGCAGCCGGGTAGTCCCGATTATGGCACAGATCGGAAGAGCGTCG30.18072289156626506No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTATTCGGAATTAC30.18072289156626506No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT30.18072289156626506No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC30.18072289156626506No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC30.18072289156626506No Hit
GTGTCAGCCGCCGCGGTAGTCCCGATTATGGCACAGATCGGCAGAGCGTC30.18072289156626506No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATCAC20.12048192771084339No Hit
GTGTCAGCAACCCCGGTAGTCCCGATTATGGCACAGATCGGAAGAGCGTC20.12048192771084339No Hit
GTGTCAGCCGCCGCGGTAAACAGGATTAGAAACCCGGGTAGTCCCGATTA20.12048192771084339No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC20.12048192771084339No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATCAC20.12048192771084339No Hit
GTGTCAGCCGCCGCGGTAAACAGGATTAGATACCCCAGTAGTCCCGATTA20.12048192771084339No Hit
GTGTCAGCCGCCGCGGTAATGCAGAGGGTGCAAGCGTTAATCGGAATTAC20.12048192771084339No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC20.12048192771084339No Hit
GTGTCAGCAGCCGCGGTAGTCCCGATTCTGGCACAGATCGGAAGAGCGTC20.12048192771084339No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC20.12048192771084339No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATGAC20.12048192771084339No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAGTTAC20.12048192771084339No Hit
GTGTCAGCAGCCGCGGTAGTCCCGATTATGGCACAGCTCGGAAGAGCGTC20.12048192771084339No Hit
GTGCCAGCAGCCGCGGTACTACAGAGGGTGCAAGCGTTAATCGGAATTAC20.12048192771084339No Hit
GTGTCAGAAACCCCGGTAGTCCCGATTATGGCACAGATCGGACGAGCGTC20.12048192771084339No Hit
GTGTCAGCCGCCGCGGTAAACAGGATTAGATACCCCTGTAGTCCCGATTA20.12048192771084339No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC20.12048192771084339No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT20.12048192771084339No Hit
GTGTCAGCAGCCGCGGTAATGCAGAGGGTGCAAGCGTTAATCGGAATTAC20.12048192771084339No Hit
GTGTCAGCCGCCGCGGTAAACAGGATTAGATACCCCCGTAGTCCCGATTA20.12048192771084339No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATGAC20.12048192771084339No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC20.12048192771084339No Hit
GTGTCAGCAGCCCGGTAGTCCCGATTATGGCACAGATCGGAAGAGCGTCG20.12048192771084339No Hit
GTGTCAGAAACCCCGGTAGTCCCGATTATGGAACAGATCGGAAGAGCGTC20.12048192771084339No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATCAC20.12048192771084339No Hit
GTGTCAGCCGCCGCGGTAGTCCCGATTATGGCACAGCTCGGACGAGCGTC20.12048192771084339No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)