FastQCFastQC Report
Tue 7 May 2024
EGAF00004969571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4011
Total Bases958.6 kbp
Sequences flagged as poor quality0
Sequence length239
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA2085.185739217152829No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1573.914235851408626No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1363.3906756419845427No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1343.3408127648965342No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1303.2410870107205185No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1233.066566940912491No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1192.966841186736475No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1172.9169783096484667No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1042.5928696085764145No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA992.468212415856395No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA972.418349538768387No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA912.2687609075043627No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA872.169035153328347No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA822.0443779606083274No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA761.894789329344303No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA731.8199950137122913No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA711.7701321366242833No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA671.6704063824482671No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA671.6704063824482671No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA611.5208177511842433No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA551.3712291199202193No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA531.3213662428322115No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA521.2964348042882072No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA370.9224632261281476No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA230.5734230865120917No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA210.5235602094240838No Hit
GGACTACCGGGGTTTCTGACACTGTGTTAACCCAAGATCGGAAGAGCACA210.5235602094240838No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA190.47369733233607575No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA170.42383445524806784No Hit
GGACTACCGGGGTTTCTGGCACTGTGTTAACCCAAGATCGGAAGAGCACA150.3739715781600598No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA130.3241087010720518No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA130.3241087010720518No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA130.3241087010720518No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA130.3241087010720518No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA130.3241087010720518No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA120.2991772625280479No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA120.2991772625280479No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA120.2991772625280479No Hit
GGACTGTGTTAACCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC110.2742458239840439Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA100.24931438544003987No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA100.24931438544003987No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA100.24931438544003987No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA100.24931438544003987No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA100.24931438544003987No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA100.24931438544003987No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA90.22438294689603588No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA90.22438294689603588No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA90.22438294689603588No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA90.22438294689603588No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA90.22438294689603588No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA70.17452006980802792No Hit
GGACTACCGCGGCTGCTGACACTGTGTTAACCCAAGATCGGAAGAGCACA70.17452006980802792No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA60.14958863126402394No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA60.14958863126402394No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA60.14958863126402394No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA60.14958863126402394No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA60.14958863126402394No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAA50.12465719272001993No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA50.12465719272001993No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTTCCCACGCTTTCGAGCCTCA50.12465719272001993No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCA50.12465719272001993No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCTTCA50.12465719272001993No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCCTCA50.12465719272001993No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCCTCA50.12465719272001993No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA50.12465719272001993No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA50.12465719272001993No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTTCCTCA50.12465719272001993No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA50.12465719272001993No Hit
GGACTACAAGGGTTTCTAATCCGATCTTACCGCGGCTGCTGACACTGTGT50.12465719272001993No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA50.12465719272001993No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA50.12465719272001993No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)