FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742
Sequences flagged as poor quality0
Sequence length239
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC33619.288174512055107No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC25014.351320321469574No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC24514.064293915040182No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC1709.758897818599312No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC412.3536165327210106No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC241.377726750861079No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC231.320321469575201No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC221.2629161882893225No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC221.2629161882893225No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC211.2055109070034442No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC191.0907003444316876No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC170.975889781859931No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC140.8036739380022963No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGGGTTAGCGTTGTTCGGAATGAC120.6888633754305395No Hit
GTGTCAGAAACCCCGGTAGTCCCAGCCTGGAGTAAGATCGGAAGAGCGTC110.6314580941446613No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT100.5740528128587831No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGGGTTAGCGTTGTTCGGAATGAC100.5740528128587831No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTACTCGGAATTAC100.5740528128587831No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC100.5740528128587831No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC100.5740528128587831No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGGGTTAGCGTTGTTCGGAATGAC90.5166475315729048No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC90.5166475315729048No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC90.5166475315729048No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC90.5166475315729048No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGGGTTAGCGTTGTTCGGAATGAC90.5166475315729048No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC80.4592422502870264No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC80.4592422502870264No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC80.4592422502870264No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTACTCGGAATTAC70.4018369690011481No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC70.4018369690011481No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC70.4018369690011481No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTAT70.4018369690011481No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC70.4018369690011481No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC70.4018369690011481No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT60.3444316877152698No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTAT60.3444316877152698No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTACTCGGAATTAC60.3444316877152698No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT60.3444316877152698No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC60.3444316877152698No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT60.3444316877152698No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT50.28702640642939153No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC50.28702640642939153No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC50.28702640642939153No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC50.28702640642939153No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC50.28702640642939153No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT50.28702640642939153No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC40.2296211251435132No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC40.2296211251435132No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT40.2296211251435132No Hit
GTCCCAGCCTGGAGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA40.2296211251435132Illumina Single End PCR Primer 1 (100% over 34bp)
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC40.2296211251435132No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTATTCGGAATTAC40.2296211251435132No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTAT40.2296211251435132No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT40.2296211251435132No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTACTCGGAATTAC40.2296211251435132No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC40.2296211251435132No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC40.2296211251435132No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC40.2296211251435132No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC30.1722158438576349No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC30.1722158438576349No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT30.1722158438576349No Hit
GTGTCAGCAGCCGCGGTAGTCCCAGCCTGGAGTAAGATCGGAAGAGCGTC30.1722158438576349No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTATTCGGAATTAC30.1722158438576349No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC30.1722158438576349No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC30.1722158438576349No Hit
GTGCCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT30.1722158438576349No Hit
GTGCCAGCCTGGAGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA30.1722158438576349Illumina Single End PCR Primer 1 (100% over 34bp)
GTGCCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTATTCGGAATTAC30.1722158438576349No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT30.1722158438576349No Hit
GTGTCAGCCGCCGCGGTAATACAGGGGGTGCAAGCGTTAATCGGAATTAC20.1148105625717566No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT20.1148105625717566No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGAATTAC20.1148105625717566No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT20.1148105625717566No Hit
GTGCCAGCAGTCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC20.1148105625717566No Hit
GTGTCAGCCGCCGCGGTAATTAGAAACCCTGGTAGTCCCAGCCTGGAGTA20.1148105625717566No Hit
GTGTCAGCCGCCGCGGTAATTAGAAACCCCCGTAGTCCCAGCCTGGAGTA20.1148105625717566No Hit
GTGCCAGCAGCCGCGGTAATTAGAAACCCGAGTAGTCCCAGCCTGGAGTA20.1148105625717566No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATCAC20.1148105625717566No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT20.1148105625717566No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC20.1148105625717566No Hit
GTGCCAGCAGCCGCGGTAATTAGATACCCGAGTAGTCCCAGCCTGGAGTA20.1148105625717566No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTAT20.1148105625717566No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAATTAGA20.1148105625717566Illumina Single End PCR Primer 1 (96% over 27bp)
GTGTCAGCAGCCGCGGTAATACAGGGGGTGCAAGCGTTAATCGGAATTAC20.1148105625717566No Hit
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAATTAGA20.1148105625717566Illumina Single End PCR Primer 1 (96% over 27bp)
GTGTCAGCCGCCGCGGTAAGACGGGGGGGGCAAGTGTTCTTCGGAATGAC20.1148105625717566No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT20.1148105625717566No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC20.1148105625717566No Hit
GTGTCAGCCGCCGCGGTAATACGCAGGGTGCAAGCGTTACTCGGAATTAC20.1148105625717566No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC20.1148105625717566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)