FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742
Sequences flagged as poor quality0
Sequence length239
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA915.223880597014925No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA874.994259471871413No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA854.879448909299656No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA784.477611940298507No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA623.5591274397244548No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA593.38691159586682No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA593.38691159586682No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA583.329506314580941No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA563.214695752009185No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA543.0998851894374284No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA512.9276693455797935No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA472.69804822043628No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA442.525832376578645No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA442.525832376578645No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA442.525832376578645No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA442.525832376578645No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA432.4684270952927667No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA412.3536165327210106No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA412.3536165327210106No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA372.123995407577497No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA321.8369690011481057No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA301.7221584385763489No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA291.6647531572904706No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA231.320321469575201No Hit
GGACTACCGGGGTTTCTGACACGCACATCGAGCAAGATCGGAAGAGCACA130.7462686567164178No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA100.5740528128587831No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA100.5740528128587831No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA80.4592422502870264No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA70.4018369690011481No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA60.3444316877152698No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACACTTTCGTGCATCA50.28702640642939153No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA50.28702640642939153No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA50.28702640642939153No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA50.28702640642939153No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA50.28702640642939153No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA50.28702640642939153No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA50.28702640642939153No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA50.28702640642939153No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA40.2296211251435132No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACACTTTCGTGCATCA40.2296211251435132No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA40.2296211251435132No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA40.2296211251435132No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA40.2296211251435132No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA40.2296211251435132No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA30.1722158438576349No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA30.1722158438576349No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA30.1722158438576349No Hit
GGACGCACATCGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC30.1722158438576349Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA30.1722158438576349No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA30.1722158438576349No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA30.1722158438576349No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA30.1722158438576349No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA30.1722158438576349No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA30.1722158438576349No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA30.1722158438576349No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA30.1722158438576349No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA30.1722158438576349No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA30.1722158438576349No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA30.1722158438576349No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA30.1722158438576349No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA30.1722158438576349No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA30.1722158438576349No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA30.1722158438576349No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA30.1722158438576349No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA30.1722158438576349No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA30.1722158438576349No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA30.1722158438576349No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA30.1722158438576349No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA30.1722158438576349No Hit
GGACTACCGCGGCTGCTGACACGCACATCGAGCAAGATCGGAAGAGCACA30.1722158438576349No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA30.1722158438576349No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGTCCCTCA30.1722158438576349No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA30.1722158438576349No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA30.1722158438576349No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA30.1722158438576349No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA30.1722158438576349No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA20.1148105625717566No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA20.1148105625717566No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACACTTTCGTGCATCA20.1148105625717566No Hit
GCACGCACATCGAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC20.1148105625717566Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA20.1148105625717566No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA20.1148105625717566No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA20.1148105625717566No Hit
GGACTACTCGGGTATCTAATCCCATTCGCTCCCCTGGCTTTCGTTCCTCA20.1148105625717566No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA20.1148105625717566No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGTCCCTCA20.1148105625717566No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA20.1148105625717566No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA20.1148105625717566No Hit
GGACTACGGGGGTTTCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG20.1148105625717566No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA20.1148105625717566No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGCACCTCA20.1148105625717566No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA20.1148105625717566No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA20.1148105625717566No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA20.1148105625717566No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA20.1148105625717566No Hit
GGACTACTCGGGTTTCTAATTACCGCGGCTGCTGGCACGCACATCGAGCA20.1148105625717566No Hit
GGACTACTCGGGTATCTAATTACCGCGGCTGCTGGCACGCACATCGAGCA20.1148105625717566No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA20.1148105625717566No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA20.1148105625717566No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA20.1148105625717566No Hit
GGACTACGGGGGTTTCTAATTACCGCGGCGGCTGACACGCACATCGAGCA20.1148105625717566No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA20.1148105625717566No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA20.1148105625717566No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA20.1148105625717566No Hit
GGACTACTGGGGTATCTAATTACCGCGGCTGCTGACACGCACATCGAGCA20.1148105625717566No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGTCCCTCA20.1148105625717566No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGTCCCTCA20.1148105625717566No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCCCCCCACGCTTTCGCACCTCA20.1148105625717566No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA20.1148105625717566No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA20.1148105625717566No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA20.1148105625717566No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA20.1148105625717566No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA20.1148105625717566No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA20.1148105625717566No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA20.1148105625717566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)