FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3758
Sequences flagged as poor quality0
Sequence length239
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC98826.290580095795633No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC67718.014901543374133No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC49313.118680149015432No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC42611.335816923895688No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC1874.976051091005854No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC1243.2996274614156467No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC992.6343799893560402No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC601.5965939329430547No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC210.5588078765300691No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC170.45236828100053217No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC150.3991484832357637No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC150.3991484832357637No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATCAC130.3459286854709952No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC110.2927088877062267No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC110.2927088877062267No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC100.2660989888238425No Hit
GTGTCAGCAGCCGCGGTACTACAGAGGGTGCAAGCGTTAATCGGAATTAC100.2660989888238425No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT100.2660989888238425No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT100.2660989888238425No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT100.2660989888238425No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATCAC100.2660989888238425No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC90.2394890899414582No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC90.2394890899414582No Hit
GTGTCAGCCGCCGCGGTAGTCCCGATTATGGCACAGATCGGAAGAGCGTC90.2394890899414582No Hit
GTGTCAGCAGCCGCGGTAGTCCCGATTATGGCACAGATCGGAAGAGCGTC80.21287919105907396No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC80.21287919105907396No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC70.18626929217668972No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC70.18626929217668972No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC60.15965939329430548No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC60.15965939329430548No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC60.15965939329430548No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATCAC60.15965939329430548No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC50.13304949441192124No Hit
GTGCCAGCAGCCGCGGTAGTCCCGATTATGGCACAGATCGGAAGAGCGTC50.13304949441192124No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTCAC50.13304949441192124No Hit
GTGCCAGCAGCCGCGGTACTACAGAGGGTGCAAGCGTTAATCGGAATTAC50.13304949441192124No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC50.13304949441192124No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTATTCGGAATTAC50.13304949441192124No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC50.13304949441192124No Hit
GTGTCAGCCGCCGCGGTACTACAGAGGGTGCAAGCGTTAATCGGAATTAC50.13304949441192124No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGAATTAC50.13304949441192124No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTCAC50.13304949441192124No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAACCGGAATTAC40.10643959552953698No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC40.10643959552953698No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAGGCGTTAATCGGAATTAC40.10643959552953698No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC40.10643959552953698No Hit
GTGCCAGCCGCCGCGGTACTACAGAGGGTGCAAGCGTTAATCGGAATTAC40.10643959552953698No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGCGCAAGCGTTAATCGGAATTAC40.10643959552953698No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC40.10643959552953698No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATCAT40.10643959552953698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)