FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1895
Sequences flagged as poor quality0
Sequence length239
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC36719.366754617414248No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC29115.356200527704486No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC21311.240105540897098No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC1437.546174142480211No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC361.8997361477572559No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC261.3720316622691293No Hit
GTGTCAGAAACCCCGGTAGTCCCAGCCTGGAGTAAGATCGGAAGAGCGTC231.2137203166226913No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC211.108179419525066No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT201.0554089709762533No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC180.9498680738786279No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC170.8970976253298154No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT170.8970976253298154No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCATTAGAAACATTAGAAACC160.8443271767810026No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC120.6332453825857519No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT100.5277044854881267No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC90.47493403693931396No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC90.47493403693931396No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC90.47493403693931396No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT80.4221635883905013No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCATTAGAAACATTAGATACC80.4221635883905013No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTATTAGAAACCCT60.31662269129287596Illumina Single End PCR Primer 1 (95% over 21bp)
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAATTAGA60.31662269129287596Illumina Single End PCR Primer 1 (96% over 27bp)
GTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC60.31662269129287596No Hit
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAATTAGA60.31662269129287596Illumina Single End PCR Primer 1 (96% over 27bp)
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCATTAGAAACATTAGAAACC60.31662269129287596No Hit
GTCCCAGCCTGGAGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA60.31662269129287596Illumina Single End PCR Primer 1 (100% over 34bp)
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCTGTAGGGAAAGAGTGT50.2638522427440633No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAATTAGA50.2638522427440633Illumina Single End PCR Primer 1 (96% over 27bp)
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTATTAGAAACCCC40.21108179419525064Illumina Single End PCR Primer 1 (95% over 21bp)
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCTAGTA40.21108179419525064No Hit
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCATTAGAAACATTAGATACC40.21108179419525064No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCCCGTA40.21108179419525064No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCATTAGAAACATTAGATACC40.21108179419525064No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCATGTAGGGAAAGAGTG40.21108179419525064Illumina Single End PCR Primer 1 (96% over 32bp)
GTGCCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC40.21108179419525064No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTATTAGATACCCT30.15831134564643798Illumina Single End PCR Primer 1 (95% over 21bp)
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC30.15831134564643798No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCATTAGAAACCCTAGTAGTC30.15831134564643798No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCGCGTA30.15831134564643798No Hit
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCATTAGAAACATTAGAAACC30.15831134564643798No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTATTCGGAATTAC30.15831134564643798No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT30.15831134564643798No Hit
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTATTAGAAACCCT30.15831134564643798Illumina Single End PCR Primer 1 (95% over 21bp)
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCCAGTA30.15831134564643798No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCGAGTA30.15831134564643798No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCATTAGATACCCCAGTA30.15831134564643798No Hit
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTATTAGATACCCT30.15831134564643798Illumina Single End PCR Primer 1 (95% over 21bp)
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG30.15831134564643798Illumina Single End PCR Primer 1 (100% over 32bp)
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCTCGTA30.15831134564643798No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGATTAGAAA30.15831134564643798Illumina Single End PCR Primer 1 (96% over 25bp)
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCTAGTA30.15831134564643798No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCTTGTA30.15831134564643798No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCCGGTA30.15831134564643798No Hit
GTGTCAGCAGCCGCGGTAGTCCCAGCCTGGAGTAAGATCGGAAGAGCGTC30.15831134564643798No Hit
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTATTAGAAACCCT30.15831134564643798Illumina Single End PCR Primer 1 (95% over 21bp)
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAATTAGA30.15831134564643798Illumina Single End PCR Primer 1 (96% over 27bp)
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCATTAGATACCCTCGTA30.15831134564643798No Hit
GTGTCAGCAGCCGCGGTAATACAGGGGGTGCAAGCGTTAATCGGAATTAC30.15831134564643798No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCATTAGATACCCCTGTA30.15831134564643798No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTATTAGAAACCCG20.10554089709762532Illumina Single End PCR Primer 1 (95% over 21bp)
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCCCGTA20.10554089709762532No Hit
GTGTCAGCAGCCGCGGTAAGATCGGATTAGAAACCCCCGTAGTCCCAGCC20.10554089709762532No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCATTAGATACCCTCGTA20.10554089709762532No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTATTAGATACCCC20.10554089709762532Illumina Single End PCR Primer 1 (95% over 21bp)
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAACCGGAATTAC20.10554089709762532No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTATTAGATACCCT20.10554089709762532Illumina Single End PCR Primer 1 (95% over 21bp)
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCTGGTA20.10554089709762532No Hit
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCTGTAGGGAAAGAGTGT20.10554089709762532No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCATTAGATACCCCCGTA20.10554089709762532No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG20.10554089709762532Illumina Single End PCR Primer 1 (100% over 32bp)
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTATTAGAAACCCG20.10554089709762532Illumina Single End PCR Primer 1 (95% over 21bp)
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTATTAGAAACCCT20.10554089709762532Illumina Single End PCR Primer 1 (95% over 21bp)
GTGCCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC20.10554089709762532No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGATTAGAAAC20.10554089709762532Illumina Single End PCR Primer 1 (95% over 23bp)
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG20.10554089709762532Illumina Single End PCR Primer 1 (100% over 32bp)
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGATTAGAAACC20.10554089709762532Illumina Single End PCR Primer 1 (95% over 22bp)
GTGTCAGCAGCCGCGGTAAGATCGGAAATTAGAAACCCTAGTAGTCCCAG20.10554089709762532No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCCTGTA20.10554089709762532No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCCTGTA20.10554089709762532No Hit
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCATTAGAAACATTAGAAACC20.10554089709762532No Hit
GGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGA20.10554089709762532Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCTGGTA20.10554089709762532No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCTTGTA20.10554089709762532No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTATTAGATACCCTAGTAG20.10554089709762532No Hit
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCATTAGAAACATTAGATACC20.10554089709762532No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGATTAGAAACCCCA20.10554089709762532No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAATTAGAAACCCCCGTAGTCCCAGC20.10554089709762532No Hit
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGATTAGAAAC20.10554089709762532Illumina Single End PCR Primer 1 (95% over 23bp)
GTGTCAGCTGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC20.10554089709762532No Hit
CTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC20.10554089709762532No Hit
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCATTAGATACCCGCGTA20.10554089709762532No Hit
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCCGGTA20.10554089709762532No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGATTAGAAACC20.10554089709762532Illumina Single End PCR Primer 1 (95% over 22bp)
GTGTCAGCAACCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC20.10554089709762532No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGATTAGATACCCCAGTAGTCC20.10554089709762532No Hit
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCGCGTA20.10554089709762532No Hit
GTGCCAGCCGCCGCGGTAAGACCGGAAGAGCGTCGTGTAGGGAAAGAGTG20.10554089709762532Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG20.10554089709762532Illumina Single End PCR Primer 1 (100% over 32bp)
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC20.10554089709762532No Hit
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCGTCATTAGATACCCTAGTA20.10554089709762532No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCGTGTA20.10554089709762532No Hit
TGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACT20.10554089709762532No Hit
GTGCCAGCAGCCGCGGTAAGATCATTAGATACCCGTGTAGTCCCAGCCTG20.10554089709762532No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGATTAGAAA20.10554089709762532Illumina Single End PCR Primer 1 (96% over 25bp)
GTGTCAGCCGCCGCGGTAAGATCATTAGAAACCCGGGTAGTCCCAGCCTG20.10554089709762532No Hit
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGGGTG20.10554089709762532Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAGGCGTTAATCGGAATTAC20.10554089709762532No Hit
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCATTAGATACCCGAGTA20.10554089709762532No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTATTAGAAACCCCAGTAG20.10554089709762532No Hit
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCGTCATTAGAAACCCCAGTA20.10554089709762532No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)