FastQCFastQC Report
Tue 7 May 2024
EGAF00004969585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1895
Total Bases452.9 kbp
Sequences flagged as poor quality0
Sequence length239
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA914.802110817941953No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA743.9050131926121368No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA633.3245382585751977No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA613.2189973614775726No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA613.2189973614775726No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA593.113456464379947No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA583.0606860158311346No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA562.955145118733509No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA542.849604221635884No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA542.849604221635884No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA522.7440633245382586No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA512.691292875989446No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA472.480211081794195No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA392.058047493403694No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA392.058047493403694No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA382.0052770448548816No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA382.0052770448548816No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA341.7941952506596308No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA341.7941952506596308No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA321.688654353562005No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA311.6358839050131926No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA271.424802110817942No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA241.2664907651715038No Hit
GGACTACCGGGGTTTCTGACACCATGTGCAAACCAGATCGGAAGAGCACA231.2137203166226913No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA170.8970976253298154No Hit
GGACCATGTGCAAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC80.4221635883905013Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGACTACTAGGGTTTCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG70.36939313984168864No Hit
GGACTACCAGGGTATCTAATGTTTCTAATGCTCTTCCGATCTTACCGCGG70.36939313984168864No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA60.31662269129287596No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA60.31662269129287596No Hit
GGACTACTAGGGTTTCTAATACACGACGCTCTTCCGATCTTACCGCGGCG50.2638522427440633Illumina Single End PCR Primer 1 (95% over 22bp)
GGACTACTGGGGTATCTAATGACGCTCTTCCGATCTTACCGCGGCGGCTG50.2638522427440633No Hit
GGACTACGCGGGTTTCTAATGTTTCTAATGCTCTTCCGATCTTACCGCGG50.2638522427440633No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA50.2638522427440633No Hit
GGACTACGGGGGTTTCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG50.2638522427440633No Hit
GGACTACCAGGGTTTCTAATGTTTCTAATGCTCTTCCGATCTTACCGCGG50.2638522427440633No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA50.2638522427440633No Hit
GGACTACTCGGGTTTCTAATGACGCTCTTCCGATCTTACCGCGGCGGCTG50.2638522427440633No Hit
GGACTACCGGGGTTTCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG50.2638522427440633No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA50.2638522427440633No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA40.21108179419525064No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA40.21108179419525064No Hit
GGACTACCAGGGTTTCTAATGACGCTCTTCCGATCTTACCGCGGCGGCTG40.21108179419525064No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA40.21108179419525064No Hit
GGACTACAAGGGTTTCTAATCCCTACACGACGCTCTTCCGATCTTACCGC40.21108179419525064Illumina Single End PCR Primer 1 (100% over 25bp)
GGACTACTGGGGTTTCTAATGTTTCTAATGCTCTTCCGATCTTACCGCGG40.21108179419525064No Hit
GGACTACAAGGGTTTCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG40.21108179419525064No Hit
GGACTACTGGGGTTTCTAATTTCCCTACACGACGCTCTTCCGATCTTACC40.21108179419525064Illumina Single End PCR Primer 1 (96% over 28bp)
GGACTACGAGGGTTTCTAATGACGCTCTTCCGATCTTACCGCGGCGGCTG40.21108179419525064No Hit
GGACTACAGGGGTATCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG40.21108179419525064No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA40.21108179419525064No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA30.15831134564643798No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA30.15831134564643798No Hit
GGACTACGCGGGTTTCTAATTTCCCTACACGACGCTCTTCCGATCTTACC30.15831134564643798Illumina Single End PCR Primer 1 (100% over 27bp)
GGACTACCGCGGCTGCTGACACCATGTGCAAACCAGATCGGAAGAGCACA30.15831134564643798No Hit
GGACTACTCGGGTATCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG30.15831134564643798No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA30.15831134564643798No Hit
GGACTACCAGGGTTTCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG30.15831134564643798No Hit
GGACTACGAGGGTTTCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG30.15831134564643798No Hit
GGACTACGCGGGTATCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG30.15831134564643798No Hit
GGACTACTAGGGTTTCTAATGCTCTTCCGATCTTACCGCGGCGGCTGACA30.15831134564643798No Hit
GGACTACAGGGGTTTCTAATGACGCTCTTCCGATCTTACCGCGGCGGCTG30.15831134564643798No Hit
GGACTACGGGGGTATCTAATGACGCTCTTCCGATCTTACCGCGGCGGCTG30.15831134564643798No Hit
GGACTACTAGGGTTTCTAATTTCCCTACACGACGCTCTTCCGATCTTACC30.15831134564643798Illumina Single End PCR Primer 1 (100% over 27bp)
GGACTACTCGGGTTTCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG30.15831134564643798No Hit
GGACTACTGGGGTTTCTAATGATACGGCGACCACCGAGATCTACACTCTT30.15831134564643798Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACACGGGTTTCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG30.15831134564643798No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA30.15831134564643798No Hit
GGACTACCAGGGTATCTAATACACGACGCTCTTCCGATCTTACCGCGGCT30.15831134564643798Illumina Single End Sequencing Primer (95% over 22bp)
GGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA30.15831134564643798No Hit
GGACTACAAGGGTATCTAATGTTTCTAATGCTCTTCCGATCTTACCGCGG30.15831134564643798No Hit
GGACTACTGGGGTTTCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG30.15831134564643798No Hit
GGACTACGAGGGTATCTAATGACGCTCTTCCGATCTTACCGCGGCGGCTG30.15831134564643798No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA30.15831134564643798No Hit
GGACTACGGGGGTTTCTAATACACGACGCTCTTCCGATCTTACCGCGGCT30.15831134564643798Illumina Single End PCR Primer 1 (95% over 22bp)
GGACTACAGGGGTTTCTAATGTTTCTAATGCTCTTCCGATCTTACCGCGG30.15831134564643798No Hit
GGACTACGCGGGTTTCTAATGACGCTCTTCCGATCTTACCGCGGCGGCTG30.15831134564643798No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA30.15831134564643798No Hit
GGACTACAGGGGTATCTAATGTTTCTAATGCTCTTCCGATCTTACCGCGG30.15831134564643798No Hit
GGACTACCCGGGTTTCTAATGATCTTACCGCGGCGGCTGACACCATGTGC20.10554089709762532No Hit
GGACTACGAGGGTTTCTAATCTCTTCCGATCTTACCGCGGCTGCTGACAC20.10554089709762532No Hit
GGACTACGGGGGTTTCTAATTCCGATCTTACCGCGGCTGCTGACACCATG20.10554089709762532No Hit
GGACTACTGGGGTATCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG20.10554089709762532No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACTTCA20.10554089709762532No Hit
GGACTACTAGGGTTTCTAATGATACGGCGACCACCGAGATCTACACTCTT20.10554089709762532Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACGGGGGTATCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG20.10554089709762532No Hit
GGACTACAGGGGTTTCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG20.10554089709762532No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA20.10554089709762532No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA20.10554089709762532No Hit
GGACTACTAGGGTATCTAATGACGCTCTTCCGATCTTACCGCGGCGGCTG20.10554089709762532No Hit
GGACTACTAGGGTATCTAATCCTGCTTGCTCCCCACGCTTTCGCACCTCA20.10554089709762532No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA20.10554089709762532No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA20.10554089709762532No Hit
GGACTACGGGGGTTTCTAATCCTACACGACGCTCTTCCGATCTTACCGCG20.10554089709762532Illumina Single End PCR Primer 1 (95% over 24bp)
GGACTACGAGGGTATCTAATGTTTCTAATGCTCTTCCGATCTTACCGCGG20.10554089709762532No Hit
GGACTACTGGGGTTTCTAATCACGACGCTCTTCCGATCTTACCGCGGCTG20.10554089709762532No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA20.10554089709762532No Hit
GGACTACCAGGGTATCTAATGACGCTCTTCCGATCTTACCGCGGCGGCTG20.10554089709762532No Hit
GGACTACGAGGGTTTCTAATGTTTCTAATGCTCTTCCGATCTTACCGCGG20.10554089709762532No Hit
GGACTACCAGGGTTTCTAATACACGACGCTCTTCCGATCTTACCGCGGCT20.10554089709762532Illumina Single End PCR Primer 1 (95% over 22bp)
GGACTACTGGGGTATCTAATCTCTTCCGATCTTACCGCGGCTGCTGACAC20.10554089709762532No Hit
GGACTACCAGGGTTTCTAATACACGACGCTCTTCCGATCTTACCGCGGCG20.10554089709762532Illumina Single End PCR Primer 1 (95% over 22bp)
GGACTACAAGGGTTTCTAATGTTTCTAATGCTCTTCCGATCTTACCGCGG20.10554089709762532No Hit
GGACTACAAGGGTATCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG20.10554089709762532No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA20.10554089709762532No Hit
GGACTACTAGGGTTTCTAATTTCCGATCTTACCGCGGCTGCTGACACCAT20.10554089709762532No Hit
GGACTACGCGGGTTTCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG20.10554089709762532No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA20.10554089709762532No Hit
GGACTACTAGGGTATCTAATGATACGGCGACCACCGAGATCTACACTCTT20.10554089709762532Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCCCA20.10554089709762532No Hit
GGACTACAAGGGTTTCTAATGATACGGCGACCACCGAGATCTACACTCTT20.10554089709762532Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACAGGGGTATCTAATGACGCTCTTCCGATCTTACCGCGGCGGCTG20.10554089709762532No Hit
GGACTACTAGGGTATCTAATACGCTCTTCCGATCTTACCGCGGCGGCTGA20.10554089709762532No Hit
GGACTACTCGGGTTTCTAATGTTTCTAATGCTCTTCCGATCTTACCGCGG20.10554089709762532No Hit
GGACTACGAGGGTTTCTAATTTCCCTACACGACGCTCTTCCGATCTTACC20.10554089709762532Illumina Single End PCR Primer 1 (100% over 27bp)
GGACTACGAGGGTATCTAATGACGCTCTTCCGATCTTACCGCGGCTGCTG20.10554089709762532No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA20.10554089709762532No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA20.10554089709762532No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA20.10554089709762532No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA20.10554089709762532No Hit
GGACTACGGGGGTTTCTAATCCGATCTTACCGCGGCTGCTGACACCATGT20.10554089709762532No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA20.10554089709762532No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCGCGCTTTCGCACCTCA20.10554089709762532No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA20.10554089709762532No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA20.10554089709762532No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA20.10554089709762532No Hit
GGACTACACGGGTATCTAATGATCTTACCGCGGCTGCTGGCACCATGTGC20.10554089709762532No Hit
GGACTACGAGGGTTTCTAATACACGACGCTCTTCCGATCTTACCGCGGCT20.10554089709762532Illumina Single End PCR Primer 1 (95% over 22bp)
GGACTACAAGGGTTTCTAATGACGCTCTTCCGATCTTACCGCGGCGGCTG20.10554089709762532No Hit
GGACTACTGGGGTTTCTAATACGCTCTTCCGATCTTACCGCGGCTGCTGA20.10554089709762532No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA20.10554089709762532No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA20.10554089709762532No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA20.10554089709762532No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA20.10554089709762532No Hit
GGACTACGGGGGTATCTAATGTTTCTAATGCTCTTCCGATCTTACCGCGG20.10554089709762532No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA20.10554089709762532No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA20.10554089709762532No Hit
GGACTACGCGGGTATCTAATGACGCTCTTCCGATCTTACCGCGGCGGCTG20.10554089709762532No Hit
GGACTACCGGGGTTTCTAATGTTTCTAATGCTCTTCCGATCTTACCGCGG20.10554089709762532No Hit
GGACTACGGGGGTTTCTAATGTTTCTAATGCTCTTCCGATCTTACCGCGG20.10554089709762532No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCA20.10554089709762532No Hit
GGACTACAGGGGTTTCTAATACACGACGCTCTTCCGATCTTACCGCGGCT20.10554089709762532Illumina Single End Sequencing Primer (95% over 22bp)
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA20.10554089709762532No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)