FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567
Sequences flagged as poor quality0
Sequence length239
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC38715.075964160498637No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC38514.998052201012854No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC32212.543825477210751No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC30511.881573821581613No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC522.025710946630308No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC481.8698870276587458No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC391.5192832099727307No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC341.3245033112582782No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAATTAGA150.584339696143358Illumina Single End PCR Primer 1 (96% over 27bp)
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAATTAGA150.584339696143358Illumina Single End PCR Primer 1 (96% over 27bp)
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT130.506427736657577No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGATTA100.38955979742890534Illumina Single End PCR Primer 1 (96% over 31bp)
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT100.38955979742890534No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC90.3506038176860148No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGATTA80.3116478379431243Illumina Single End PCR Primer 1 (96% over 31bp)
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAATTAGA80.3116478379431243Illumina Single End PCR Primer 1 (96% over 27bp)
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGATTA70.2726918582002337Illumina Single End PCR Primer 1 (96% over 31bp)
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT70.2726918582002337No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGATTAGATA70.2726918582002337Illumina Single End PCR Primer 1 (96% over 25bp)
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAATTAGA70.2726918582002337Illumina Single End PCR Primer 1 (96% over 27bp)
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT70.2726918582002337No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC70.2726918582002337No Hit
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGATTAGAAA60.23373587845734323Illumina Single End PCR Primer 1 (96% over 25bp)
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGATTA60.23373587845734323Illumina Single End PCR Primer 1 (96% over 31bp)
GTGCCAGCCTGGAGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA60.23373587845734323Illumina Single End PCR Primer 1 (100% over 34bp)
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGATTTAC60.23373587845734323No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGATTAGAAA60.23373587845734323Illumina Single End PCR Primer 1 (96% over 25bp)
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGATTTAC50.19477989871445267No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT50.19477989871445267No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGATTAGAAAC50.19477989871445267Illumina Single End PCR Primer 1 (95% over 23bp)
GTGCCAGCAGCCGCGGTAAGATCATTAGAAACCCCCGTAGTCCCAGCCTG50.19477989871445267No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGATTTAC50.19477989871445267No Hit
GTGCCAGCCGCCGCGGTAAGATCGGAATTAGATACCCCCGTAGTCCCAGC50.19477989871445267No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT50.19477989871445267No Hit
GTGCCAGCCGCCGCGGTAAGCAGAAGACGGCATACGAGATGTGACTGGAG40.15582391897156214Illumina PCR Primer Index 11 (96% over 26bp)
GTGTCAGCCGCCGCGGTAAGATCGGAATTAGAAACCCTGGTAGTCCCAGC40.15582391897156214No Hit
GTGTCAGCCGCCGCGGTAAAGACGGCATACGAGATGTGACTGGAGTTCAG40.15582391897156214TruSeq Adapter, Index 1 (95% over 21bp)
GTGTCAGCAGCCGCGGTAAGATCGGAAGATTAGAAACCCGCGTAGTCCCA40.15582391897156214No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGATTAGATA40.15582391897156214Illumina Single End PCR Primer 1 (96% over 25bp)
GTGCCAGCCGCCGCGGTAAAGACGGCATACGAGATGTGACTGGAGTTCAG40.15582391897156214TruSeq Adapter, Index 1 (95% over 21bp)
GTGTCAGCCGCCGCGGTAAGATCGGAATTAGAAACCCGAGTAGTCCCAGC40.15582391897156214No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGATTAGAAA40.15582391897156214Illumina Single End PCR Primer 1 (96% over 25bp)
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGATTAGATA40.15582391897156214Illumina Single End PCR Primer 1 (96% over 25bp)
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAACCGGAATTAC40.15582391897156214No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC40.15582391897156214No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTATTCGGAATTAC40.15582391897156214No Hit
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGATTAGAAA40.15582391897156214Illumina Single End PCR Primer 1 (96% over 25bp)
GTGCCAGCAGCCGCGGTAAGATCATTAGATACCCGCGTAGTCCCAGCCTG30.11686793922867161No Hit
GTGCCAGCAGCCGCGGTAAAGACGGCATACGAGATGTGACTGGAGTTCAG30.11686793922867161TruSeq Adapter, Index 1 (95% over 21bp)
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC30.11686793922867161No Hit
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGGATTAGATA30.11686793922867161Illumina Single End PCR Primer 1 (96% over 25bp)
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC30.11686793922867161No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC30.11686793922867161No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGATTAGATACCCGGGTAGTCCCA30.11686793922867161No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGATTAGAAACCCGTGTAGTCCCA30.11686793922867161No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAATTAGAAACCCTAGTAGTCCCAGC30.11686793922867161No Hit
GTGTCAGCAGCCGCGGTAAGATCATTAGATACCCGCGTAGTCCCAGCCTG30.11686793922867161No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTATTCGGAATTAC30.11686793922867161No Hit
GTGTCAGCAGCCGCGGTAAGCAGAAGACGGCATACGAGATGTGACTGGAG30.11686793922867161Illumina PCR Primer Index 11 (96% over 26bp)
GTGTCAGCAGCCGCGGTAAGATCGGAATTAGAAACCCCCGTAGTCCCAGC30.11686793922867161No Hit
GTGCCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGGATTAGAAAC30.11686793922867161Illumina Single End PCR Primer 1 (95% over 23bp)
GTGTCAGCCGCCGCGGTAAGATCGGATTAGAAACCCGCGTAGTCCCAGCC30.11686793922867161No Hit
GTGCCAGCAGCCGCGGTAAGATCGGAATTAGATACCCCCGTAGTCCCAGC30.11686793922867161No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGATTAGATACCCCCGTAGTCCCA30.11686793922867161No Hit
GTGCCAGCAGCCGCGGTAAGATCGGAAGATTAGAAACCCCTGTAGTCCCA30.11686793922867161No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC30.11686793922867161No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGATTAGAAACCCTAGTAGTCCCA30.11686793922867161No Hit
GTGTCAGCAGCCGCGGTAAGATCGGAAGAGCGTCGTGTAGATTAGAAACC30.11686793922867161Illumina Single End PCR Primer 1 (95% over 22bp)
GTGCCAGCCGCCGCGGTAAGATCGGAAGAGCGTCGTGTATTAGAAACCCG30.11686793922867161Illumina Single End PCR Primer 1 (95% over 21bp)
GTGTCAGCCGCCGCGGTAAGATCGGAAGATTAGATACCCCGGTAGTCCCA30.11686793922867161No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC30.11686793922867161No Hit
GTGTCAGCAGCCGCGGTAAAGACGGCATACGAGATGTGACTGGAGTTCAG30.11686793922867161TruSeq Adapter, Index 1 (95% over 21bp)
GTGTCAGCAGCCGCGGTAAGATCATTAGAAACCCGTGTAGTCCCAGCCTG30.11686793922867161No Hit
GTGTCAGCCGCCGCGGTAAGATCGGAAGATTAGATACCCGCGTAGTCCCA30.11686793922867161No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)