FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567
Sequences flagged as poor quality0
Sequence length239
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1164.518893650175302No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA903.506038176860148No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA883.428126217374367No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA833.2333463186599145No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA803.1164783794312427No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA772.999610440202571No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA772.999610440202571No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA752.92169848071679No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA752.92169848071679No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA732.8437865212310087No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA682.6490066225165565No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA672.6100506427736656No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA642.4931827035449943No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA642.4931827035449943No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA612.3763147643163225No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA612.3763147643163225No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA582.259446825087651No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA562.18153486560187No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA552.1425788858589794No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA542.103622906116089No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA532.0646669263731985No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA522.025710946630308No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA522.025710946630308No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA501.9477989871445267No Hit
GGACTACGGGGGTTTCTAATTTCCCTACACGACGCTCTTCCGATCTTACC80.3116478379431243Illumina Single End PCR Primer 1 (96% over 28bp)
GCACCTTCCGTGTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC70.2726918582002337Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGACTACGAGGGTTTCTAATTTCCCTACACGACGCTCTTCCGATCTTACC60.23373587845734323Illumina Single End PCR Primer 1 (100% over 27bp)
GGACTACGGGGGTTTCTAATCCCTACACGACGCTCTTCCGATCTTACCGC50.19477989871445267Illumina Single End PCR Primer 1 (96% over 26bp)
GGACTACTGGGGTTTCTAATTTCCCTACACGACGCTCTTCCGATCTTACC50.19477989871445267Illumina Single End PCR Primer 1 (96% over 28bp)
GGACTACGGGGGTATCTAATTCCGATCTTACCGCGGCGGCTGGCACCTTC40.15582391897156214No Hit
GGACTACGCGGGTTTCTAATTTCCCTACACGACGCTCTTCCGATCTTACC40.15582391897156214Illumina Single End PCR Primer 1 (100% over 27bp)
GGACTACCCGGGTATCTAATCTTCCGATCTTACCGCGGCGGCTGACACCT40.15582391897156214No Hit
GGACTACAAGGGTTTCTAATCCCTACACGACGCTCTTCCGATCTTACCGC40.15582391897156214Illumina Single End PCR Primer 1 (100% over 25bp)
GGACTACCAGGGTATCTAATCCCTACACGACGCTCTTCCGATCTTACCGC40.15582391897156214Illumina Single End Sequencing Primer (96% over 26bp)
GGACTACGGGGGTTTCTAATGATCTTACCGCGGCTGCTGGCACCTTCCGT40.15582391897156214No Hit
GGACTACGCGGGTTTCTAATCTTCCGATCTTACCGCGGCTGCTGACACCT40.15582391897156214No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA40.15582391897156214No Hit
GGACTACTCGGGTTTCTAATTCCGATCTTACCGCGGCGGCTGACACCTTC40.15582391897156214No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA30.11686793922867161No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA30.11686793922867161No Hit
GGACTACGGGGGTATCTAATTCCGATCTTACCGCGGCTGCTGGCACCTTC30.11686793922867161No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA30.11686793922867161No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA30.11686793922867161No Hit
GGACTACGCGGGTTTCTAATCTTTCCCTACACGACGCTCTTCCGATCTTA30.11686793922867161Illumina Single End Sequencing Primer (96% over 31bp)
GGACTACTCGGGTTTCTAATTTCCCTACACGACGCTCTTCCGATCTTACC30.11686793922867161Illumina Single End PCR Primer 1 (100% over 27bp)
GGACTACACGGGTTTCTAATTTCCCTACACGACGCTCTTCCGATCTTACC30.11686793922867161Illumina Single End PCR Primer 1 (96% over 28bp)
GGACTACGGGGGTATCTAATCTTCCGATCTTACCGCGGCGGCTGACACCT30.11686793922867161No Hit
GGACTACCGGGGTATCTAATTTCCCTACACGACGCTCTTCCGATCTTACC30.11686793922867161Illumina Single End Sequencing Primer (96% over 28bp)
GGACTACGGGGGTTTCTAATTCCGATCTTACCGCGGCTGCTGACACCTTC30.11686793922867161No Hit
GGACTACGCGGGTATCTAATCTTCCGATCTTACCGCGGCGGCTGACACCT30.11686793922867161No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA30.11686793922867161No Hit
GGACTACGGGGGTTTCTAATTCCGATCTTACCGCGGCGGCTGACACCTTC30.11686793922867161No Hit
GGACTACGGGGGTTTCTAATCTTCCGATCTTACCGCGGCTGCTGGCACCT30.11686793922867161No Hit
GGACTACGCGGGTTTCTAATCCGATCTTACCGCGGCGGCTGACACCTTCC30.11686793922867161No Hit
GGACTACCAGGGTTTCTAATCTTCCGATCTTACCGCGGCGGCTGACACCT30.11686793922867161No Hit
GGACTACAGGGGTTTCTAATCTTCCGATCTTACCGCGGCTGCTGGCACCT30.11686793922867161No Hit
GGACTACCCGGGTTTCTAATCGGAAGAGCACACGTCTGAACTCCAGTCAC30.11686793922867161Illumina Multiplexing Index Sequencing Primer (96% over 33bp)
GGACTACACGGGTTTCTAATCTTCCGATCTTACCGCGGCTGCTGACACCT30.11686793922867161No Hit
GGACTACTAGGGTTTCTAATTTCCCTACACGACGCTCTTCCGATCTTACC30.11686793922867161Illumina Single End PCR Primer 1 (100% over 27bp)
GGACTACTAGGGTTTCTAATCTTCCGATCTTACCGCGGCGGCTGACACCT30.11686793922867161No Hit
GGACTACGCGGGTATCTAATGATCTTACCGCGGCTGCTGGCACCTTCCGT30.11686793922867161No Hit
GGACTACTGGGGTTTCTAATCTTTCCCTACACGACGCTCTTCCGATCTTA30.11686793922867161Illumina Single End PCR Primer 1 (96% over 31bp)
GGACTACGGGGGTTTCTAATCGGAAGAGCACACGTCTGAACTCCAGTCAC30.11686793922867161Illumina Multiplexing Index Sequencing Primer (96% over 33bp)
GGACTACCCGGGTTTCTAATCTTTCCCTACACGACGCTCTTCCGATCTTA30.11686793922867161Illumina Single End PCR Primer 1 (96% over 31bp)
GGACTACCAGGGTATCTAATCTTCCGATCTTACCGCGGCTGCTGGCACCT30.11686793922867161No Hit
GGACTACGCGGGTTTCTAATCCTACACGACGCTCTTCCGATCTTACCGCG30.11686793922867161Illumina Single End Sequencing Primer (95% over 24bp)
GGACTACCGGGGTATCTAATCTTCCGATCTTACCGCGGCGGCTGACACCT30.11686793922867161No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCCTTCGCACCTCA30.11686793922867161No Hit
GGACTACGCGGGTATCTAATCCCTACACGACGCTCTTCCGATCTTACCGC30.11686793922867161Illumina Single End Sequencing Primer (96% over 26bp)
GGACTACAGGGGTTTCTAATCGGAAGAGCACACGTCTGAACTCCAGTCAC30.11686793922867161Illumina Multiplexing Index Sequencing Primer (96% over 33bp)
GGACTACTAGGGTATCTAATCTTTCCCTACACGACGCTCTTCCGATCTTA30.11686793922867161Illumina Single End Sequencing Primer (96% over 31bp)
GGACTACGCGGGTATCTAATGATCTTACCGCGGCTGCTGACACCTTCCGT30.11686793922867161No Hit
GGACTACAAGGGTATCTAATCTTTCCCTACACGACGCTCTTCCGATCTTA30.11686793922867161Illumina Single End Sequencing Primer (96% over 31bp)
GGACTACACGGGTTTCTAATGATCTTACCGCGGCTGCTGACACCTTCCGT30.11686793922867161No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)