FastQCFastQC Report
Tue 7 May 2024
EGAF00004969590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12784
Total Bases3 Mbp
Sequences flagged as poor quality0
Sequence length239
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC234818.366708385481854No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC221017.287234042553195No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC192915.089173967459324No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC163212.76595744680851No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC2742.1433041301627034No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC2541.9868585732165207No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC2401.877346683354193No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC1831.431476846057572No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC1230.9621401752190237No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC1190.9308510638297872No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT1040.8135168961201502No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT1010.7900500625782227No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC980.7665832290362953No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC930.7274718397997497No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC910.7118272841051314No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC850.6648936170212766No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC800.6257822277847309No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT780.6101376720901126No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC780.6101376720901126No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT710.5553817271589487No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC630.4928035043804756No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC600.46933667083854824No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT580.4536921151439299No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT570.4458698372966208No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC570.4458698372966208No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC550.4302252816020025No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC500.39111389236545685No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT490.38329161451814764No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC450.3520025031289111No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC440.344180225281602No Hit
GTGCCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT370.28942428035043805No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC340.26595744680851063No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT340.26595744680851063No Hit
GTGCCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT330.2581351689612015No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT320.2503128911138924No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT290.22684605757196494No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT240.18773466833541927No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC230.17991239048811014No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC230.17991239048811014No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT210.16426783479349186No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC210.16426783479349186No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT200.15644555694618273No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT190.1486232790988736No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC160.1251564455569462No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC150.11733416770963706No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC150.11733416770963706No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC150.11733416770963706No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC140.1095118898623279No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGATTTAT140.1095118898623279No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC130.10168961201501878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)