FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12174973
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG75500.011.16597594-95
CGTATGC98250.010.63494794-95
CTCGTAT120350.09.70809994-95
TCGTATG107900.09.573770594-95
TCTCGTA137550.09.44369594-95
GTATGCC107100.09.26834294-95
ATGCCGT79650.08.70587294-95
ATCTCGT160150.08.3779394-95
AATCTCG170800.08.20312694-95
GCCGTCT75400.07.968276594-95
TCGAATC209650.07.872364594-95
TTCGAAT230950.07.76326294-95
CCGTCTT72200.07.53205694-95
CTTCGAA270700.07.526867494-95
ACTTCGA296050.07.387708794-95
CGAATCT195650.07.36756794-95
GTATACG6550.00356564347.3374411
CACTTCG322150.07.106145494-95
TGCCGTC82600.06.957457594-95
GAATCTC226550.06.446533794-95
Produced by FastQC (version 0.11.5)