FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15694367
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC74450.09.98325494-95
TATGCCG60150.09.47475794-95
TCGTATG80100.09.45694194-95
CTCGTAT89200.08.67851294-95
TCTCGTA103950.08.56641894-95
ATCTCGT123000.07.818841594-95
ATGCCGT74100.07.659002394-95
GTATGCC88200.07.29614194-95
CATCTCG159550.06.59324894-95
ATTGGCT298350.06.17629794-95
ACATTGG336750.05.85984294-95
CATTGGC332550.05.79817894-95
TGCCGTC83150.05.625957594-95
TTGGCTC320650.05.598646694-95
CACATTG377800.05.55626394-95
GTCACAT407750.05.485919594-95
GCTCATC265050.05.357545494-95
AGTCACA466550.05.308583394-95
CAGTCAC511650.05.26299194-95
GGCTCAT282700.05.1742594-95
Produced by FastQC (version 0.11.5)