FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12186712
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG165950.011.2047894-95
CGTATGC212250.010.69618994-95
ATGCCGT159500.010.48167894-95
GCCGTCT136200.010.11276194-95
GTATGCC206850.09.98809394-95
TCGTATG236450.09.98311194-95
CCGTCTT118900.09.84654694-95
TGCCGTC155950.09.83707494-95
CTCGTAT262900.09.51166694-95
AATCTCG362000.09.17757694-95
TCTCGTA292450.08.9484794-95
ATCTCGT337650.08.81961594-95
TAATCTC430800.08.02609994-95
CGTCTTC131550.07.79851294-95
CTTAATC488650.07.74656794-95
TTAATCT460250.07.739560694-95
ACTTAAT538050.07.432557694-95
CGAACTT682100.07.09188894-95
AACTTAA607000.06.936475894-95
CCGAACT737300.06.88946494-95
Produced by FastQC (version 0.11.5)