FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11728090
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA124500.011.78572494-95
CCGTATC143350.011.196694-95
CGCCGTA183800.011.1481494-95
GCCGTAT161300.011.0987594-95
GTATCAT127550.010.59177994-95
TCGCCGT213650.010.17957694-95
GTCGCCG247200.09.56638894-95
GGTCGCC280650.09.22143694-95
TATCATT134900.09.20506694-95
TGGTCGC303100.09.15726394-95
GTGGTCG347650.08.45503794-95
ATCATTA123100.08.31297294-95
CGGTGGT433750.07.783899394-95
TCATTAA118100.07.478769394-95
GGTGGTC444450.07.42021494-95
TCGGTGG465850.07.323991394-95
GATCTCG635600.07.220796694-95
ATCTCGG590850.07.040345794-95
TCTCGGT537950.07.03090194-95
CTCGGTG518400.06.85636594-95
Produced by FastQC (version 0.11.5)