FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13912547
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA49550.010.73039294-95
GCCGTAT62650.010.13050594-95
CGCCGTA70950.010.025599594-95
CCGTATC58450.09.50626294-95
GTCGCCG106850.08.06927194-95
TGGTCGC121600.08.0358794-95
TCGCCGT89650.07.907655294-95
GGTCGCC120250.07.80741594-95
TCTCGGT217550.07.28794794-95
GTGGTCG141650.07.20276694-95
ATCTCGG246550.06.72213794-95
GATCTCG261000.06.707847694-95
CGGTGGT190800.06.52726594-95
TCGGTGG196800.06.133547394-95
CTCGGTG230500.06.068036694-95
TAGATCT292900.05.961022492-93
AGATCTC315950.05.882333394-95
GTATCAT73950.05.82962594-95
GAGTGTA395900.05.74099394-95
AGTGTAG381650.05.672956594-95
Produced by FastQC (version 0.11.5)