FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23494658
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAG416200.06.5381471
CCAAAGT346150.06.30956942
CCCGAGT94250.06.2342871
CCCTAAA110200.06.2060471
CCCAAGT193250.05.90659481
CGCCGTA26650.05.698519794-95
CCGAGTA59550.05.66086532
CCCAAAA201600.05.6380611
CCCAAAT170300.05.62790441
AAAGTGC299750.05.60625174
CCCCGAT48450.05.5667611
CCCGAAT31301.8189894E-125.5394481
CGATAGT13055.223653E-45.53591061
CCCTAAT67000.05.46321
CAAAGTG361400.05.43791533
CCCAATT91100.05.39249941
CCCGAAA42150.05.37042471
CGTCGAA15451.6077844E-45.2994251
AAGTGCT337550.05.159725
CGAGTAG55900.05.0951823
Produced by FastQC (version 0.11.5)