FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13438310
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG61650.010.20802894-95
TCGTATG82550.09.46472394-95
CGTATGC74000.09.04995594-95
CTCGTAT90100.08.61890494-95
ATCTCGT123450.08.09878994-95
AATCTCG130250.07.876532694-95
ATGCCGT68450.07.875560894-95
TCTCGTA104600.07.855493594-95
GTATGCC87950.07.398501494-95
CTAAATC183700.06.735310694-95
GCCTAAA218050.06.51289694-95
TGCCGTC78800.06.41922394-95
GCCGTCT71550.06.14032294-95
TGCCTAA237500.06.129512394-95
CCTAAAT210350.05.927149394-95
TAAATCT202950.05.920938594-95
ATGCCTA263000.05.68871194-95
ACATGCC325900.05.661946394-95
CCGTCTT68850.05.55329894-95
AAATCTC208150.05.248201494-95
Produced by FastQC (version 0.11.5)