FastQCFastQC Report
Fri 9 Sep 2022
EGAF00004970861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20116
Sequences flagged as poor quality0
Sequence length66-26784
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC5952.957844501889044No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCT3991.9834957247961822No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGTTCATGCTC1390.6909922449791212No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACGAGTTCATGCT1040.5170013919268244No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAGTTCATGCTCT1020.5070590574666932No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCACAGAGTTCATG910.4523762179359713No Hit
AGTAACTGAAAATGCTTCTGAGTGGCTAGGATAACAGATGTGCACCACCA800.3976933784052496No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGT790.39272221117518397No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTC650.32312586995426523No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACGAGTTCATGCTC580.2883276993438059No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAGCAATCTTCCCAAGGT570.28335653211374034No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGTTCATGCTC560.27838536488367466No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCCTGAGTTCATGCTC510.2535295287333466No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCC510.2535295287333466No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTC510.2535295287333466No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCCTGAGTTCATGCT510.2535295287333466No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAGCAATCTTCCCAAGGTC450.22370252535295287No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAGCAATCTTCCCAAGGTC410.2038178564326904No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGATTCATGCTCT410.2038178564326904No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCACAGAGTTCATGCT400.1988466892026248No Hit
TTGAAGAGAGGTGACAAAACAAAGGAAATGCAGAGGAGACCTTGACATAA360.1789620202823623No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTC360.1789620202823623No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGTTCATGCTCT300.1491350169019686No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGGATTCATGC300.1491350169019686No Hit
AGTAACTGAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT280.13919268244183733No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAAGTTCATGCT280.13919268244183733No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAGCAATCTTCCCAAGGTC270.13422151521177172No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAAGTTCATGCTC240.11930801352157487No Hit
AGTAACTGAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCTC230.11433684629150924No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATAC230.11433684629150924No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAAGGTC230.11433684629150924No Hit
AGTAACTGAAAATGCTTCTGATGGCAGACAGCTGTCACAGAGTTCATGCT220.10936567906144362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)