FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004970865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24210
Sequences flagged as poor quality0
Sequence length29-32341
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC6432.6559273027674513No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCT4952.0446096654275094No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGT1730.714580751755473No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGTTCATGCTC1490.6154481619165635No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTC1490.6154481619165635No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAGTTCATGCTCT1400.5782734407269724No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACGAGTTCATGCT1340.553490293267245No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTC1310.5410987195373812No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCACAGAGTTCATG1130.4667492771581991No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACGAGTTCATGCTC1030.4254440313919867No Hit
AGTAACTGAAAATGCTTCTGAGTGGCTAGGATAACAGATGTGCACCACCA900.37174721189591076No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCC740.3056588186699711No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTC660.27261462205700127No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCCTGAGTTCATGCT590.24370095002065265No Hit
TTGAAGAGAGGTGACAAAACAAAGGAAATGCAGAGGAGACCTTGACATAA530.21891780256092525No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTCC520.214787277984304No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCCTGAGTTCATGCTC510.21065675340768275No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGTTCATGCTC500.20652622883106153No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCT460.19000413052457663No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTCCT460.19000413052457663No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCACAGAGTTCATGCT450.18587360594795538No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCC420.1734820322180917No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTCCT390.161090458488228No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCCAACAATCTTCCCAAGGTCC390.161090458488228No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAAGGT380.15695993391160679No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAAGGT370.15282940933498554No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAAGGTC360.14869888475836432No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGTTCATGCTCT340.14043783560512185No Hit
AGTAACTGAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT340.14043783560512185No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAAGG330.13630731102850063No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGATTCATGCTCT300.12391573729863693No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCAACAATCTTCCCAAGGTCCT300.12391573729863693No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGGATTCATGC290.1197852127220157No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCCTG280.11565468814539447No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATAC280.11565468814539447No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCTGAGTTCATGCT280.11565468814539447No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTCTG270.11152416356877323No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGATTCATGCTCTC270.11152416356877323No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTCTG270.11152416356877323No Hit
TTGAAGAGGTGACAAAACAAAGGAAATGCAGAGGAGACCTTGACATAAAG250.10326311441553077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)