FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004970876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13392
Sequences flagged as poor quality0
Sequence length51-26730
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC5073.7858422939068106No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCT2231.6651732377538828No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGTTCATGCTC1070.798984468339307No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTC1030.769115890083632No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGT850.6347072879330944No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACGAGTTCATGCT820.6123058542413381No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTC750.5600358422939068No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAGTTCATGCTCT640.4778972520908005No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCACAGAGTTCATG540.4032258064516129No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCC530.39575866188769415No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTC480.35842293906810035No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACGAGTTCATGCTC410.30615292712066905No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCCTGAGTTCATGCT400.2986857825567503No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTCC390.29121863799283154No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGTTCATGCTC360.2688172043010753No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTCCT320.23894862604540024No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCC320.23894862604540024No Hit
AGTAACTGAAAATGCTTCTGAGTGGCTAGGATAACAGATGTGCACCACCA310.23148148148148145No Hit
AGTAACTGAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT290.21654719235364395No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCT290.21654719235364395No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCACAGAGTTCATGCT250.18667861409796893No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGTTCATGCTCT250.18667861409796893No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCCAACAATCTTCCCAAGGTCC230.17174432497013142No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATAC230.17174432497013142No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTCCT230.17174432497013142No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAAGGT220.16427718040621267No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCCTGAGTTCATGCTC220.16427718040621267No Hit
TTGAAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCCTG220.16427718040621267No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTCTG210.15681003584229392No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAAGG210.15681003584229392No Hit
TTGAAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTCCT200.14934289127837516No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTCT190.1418757467144564No Hit
TTGAAGAGAGGTGACAAAACAAAGGGGAGTATCCAACAATCTTCCCAAGG180.13440860215053765No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCAACAATCTTCCCAAGGTCCT180.13440860215053765No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAAGGTC180.13440860215053765No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCT170.12694145758661887No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCCTG170.12694145758661887No Hit
TTGAAGAGAGGTGACAAAACAAAGGAAATGCAGAGGAGACCTTGACATAA140.1045400238948626No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGAGTTCATGC140.1045400238948626No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTCTG140.1045400238948626No Hit
GGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC140.1045400238948626No Hit
TTGAAGAGAGGTGACAAAACAAAGGGGAGTATCAACAATCTTCCCAAGGT140.1045400238948626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)