FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004970878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18193
Sequences flagged as poor quality0
Sequence length27-25556
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC5843.2100258341120207No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCT3301.8138844610564502No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGTTCATGCTC1320.7255537844225801No Hit
AGTAACTGAAAATGCTTCTGAGTGGCTAGGATAACAGATGTGCACCACCA1130.6211180124223602No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAGTTCATGCTCT1060.5826416753696477No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACGAGTTCATGCT820.4507228054746331No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACGAGTTCATGCTC710.39025999010608475No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCACAGAGTTCATG710.39025999010608475No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAGCAATCTTCCCAAGGTC590.3243005551585775No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAGCAATCTTCCCAAGGT480.2638377397900291No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCCTGAGTTCATGCT480.2638377397900291No Hit
TTGAAGAGAGGTGACAAAACAAAGGAAATGCAGAGGAGACCTTGACATAA390.21436816357939867No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAGCAATCTTCCCAAGGTC360.19787830484252183No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGT350.19238168526356292No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTC330.18138844610564503No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCACAGAGTTCATGCT290.15940196778980925No Hit
AGTAACTGAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT280.15390534821085033No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATAC260.14291210905293245No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAGCAATCTTCCCAAGGTCC260.14291210905293245No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTC260.14291210905293245No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGTTCATGCTC250.1374154894739735No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAGCAATCTTCCCAAGGTC250.1374154894739735No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCCTGAGTTCATGCTC240.13191886989501456No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGCAAAGGGAAATGCAGAGGAGACC230.12642225031605564No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGTTCATGCTCT220.1209256307370967No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGGTTCATGCT220.1209256307370967No Hit
AGTAACTGAAAATGCTTCTAGTGGCTAGGATAACAGATGTGCACCACCAT210.11542901115813775No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAGCAATCTTCCCAAGGTCCT200.1099323915791788No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAGCAATCTTCCCAAGGTCC200.1099323915791788No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCC190.10443577200021986No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGATTCATGCTCT190.10443577200021986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)