FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004970903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15137
Sequences flagged as poor quality0
Sequence length42-33223
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAAT6314.168593512585057No Hit
CTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAATC2151.4203607055559226No Hit
CCTGAAGAAATGTCACGGCTTGTTGGCGCAGGCCGCCTGCTCTCAGGAAT2071.367510074651516No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGTAGGGAACTTGGC2071.367510074651516No Hit
GCCATGACCATTGTTATTTGTCAGAGGTCAAAATTGTAGGGAACTTGGCC1090.7200898460725376No Hit
CTGAAGAAATGTCACGGCTTGTTGGCGCAGGCCGCCTGCTCTCAGGAATC900.5945695976745723No Hit
CCCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAA850.5615379533593182No Hit
GCCATGACCATTATTATTTGTCCAGAGGTCAAAATTGTAGGGAACTTGGC570.37656074519389576No Hit
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAAGGAA510.33692277201559095No Hit
CCCTGAAGAAATGTCACGGCTTGTTGGCGCAGGCCGCCTGCTCTCAGGAA400.26425315452203213No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGTGAGAACTTGGCC390.2576468256589813No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGGCAGGGAACTTGG350.23122151020677806No Hit
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGAATC340.2246151813437273No Hit
GCCATGACCATTATTTGTCCAGAGGTCAAAATTGTAGGGAACTTGGCCAC320.21140252361762568No Hit
GCCATGACCATTGTTGTCCAGAGGTCAAAATTGTAGGGAACTTGGCCACC310.2047961947545749No Hit
CCTGAAGAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAATC310.2047961947545749No Hit
CCTGAAGAAATGTCGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAATCT300.19818986589152407No Hit
GCCATGACCGTGTTATTTGTCCAGAGGTCAAAATTGTAGGGAACTTGGCC290.19158353702847328No Hit
CCTGAAGAAATGTCACGGCTTGTTGGCGCAGGCCGCCTGCTCTCAAGGAA270.17837087930237167No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGTAGGAACTTGGCC260.17176455043932087No Hit
GCCATGACCATTATTATTTGTCAGAGGTCAAAATTGTAGGGAACTTGGCC230.15194556385016844No Hit
CTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAAGGAAT230.15194556385016844No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGTAGAACTTGGCCA230.15194556385016844No Hit
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCCTCAGGAA210.13873290612406686No Hit
CCTGAAGAAATGTCACGGCTTGTTAAGCGCAGGCCGCCTGCTCTCAGGAA190.12552024839796525No Hit
CCTGAAGGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAA190.12552024839796525No Hit
GCCATGACCATTGTTATTTGTCAGAGGTCAAAATTGTGAGAACTTGGCCA180.11891391953491445No Hit
CTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGAATCT180.11891391953491445No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAATTGTAGGGAACTTGGCC170.11230759067186365No Hit
CCTGAAGAAATGTACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAATC170.11230759067186365No Hit
GCCATGACCGTATTATTTGTCCAGAGGTCAAAATTGTAGGGAACTTGGCC170.11230759067186365No Hit
CCTGAAGAAATGTCACGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAATC170.11230759067186365No Hit
TGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAATCT160.10570126180881284No Hit
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCGCCTGCTCTCAGGAATC160.10570126180881284No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGGCAGAACTTGGCC160.10570126180881284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)