FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004970905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10399
Sequences flagged as poor quality0
Sequence length70-26017
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC2632.52908933551303No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCT2482.384844696605443No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGT780.7500721223194537No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTC730.7019905760169247No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGTTCATGCTC660.634676411193384No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTC620.5962111741513607No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAGTTCATGCTCT550.52889700932782No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACGAGTTCATGCT450.43273391672276185No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCC440.42311760746225596No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACGAGTTCATGCTC360.34618713337820944No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCCTGAGTTCATGCT350.3365708241177036No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCACAGAGTTCATG330.317338205596692No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTC290.27887296855466875No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCC260.25002404077315127No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCACAGAGTTCATGCT230.2211751129916338No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCCTG230.2211751129916338No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTCCT230.2211751129916338No Hit
TTGAAGAGAGGTGACAAAACAAAGGAAATGCAGAGGAGACCTTGACATAA200.19232618521011635No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCT190.18270987594961055No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTCC190.18270987594961055No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGATTCATGCTCT180.17309356668910472No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCCAACAATCTTCCCAAGGTCC180.17309356668910472No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGATTCATGCTCTC180.17309356668910472No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGTTCATGCTC170.1634772574285989No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCCTGAGTTCATGCTC170.1634772574285989No Hit
AGTAACTGAAAATGCTTCTGAGTGGCTAGGATAACAGATGTGCACCACCA170.1634772574285989No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTCCT150.14424463890758726No Hit
AGTAACTGAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT150.14424463890758726No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCTGAGTTCATGCT150.14424463890758726No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAAGGT150.14424463890758726No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAAGTTCATGCTC140.13462832964708144No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAAGG130.12501202038657563No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAAGGT120.1153957111260698No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCAACAATCTTCCCAAGGTCCT120.1153957111260698No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGTTCATGCTCT120.1153957111260698No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAAGGTC120.1153957111260698No Hit
AGTAACTGAAAATGCTTCTGATGGCAGACAGCTGTCACAGAGTTCATGCT120.1153957111260698No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATACT120.1153957111260698No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTCTG110.10577940186556399No Hit
TTGAAGAGAGGTGACAAAACAAAGGGTATCAACAATCTTCCCAAGGTCCT110.10577940186556399No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAAGTTCATGCT110.10577940186556399No Hit
TTGAAGAGAGGTGACAAAACAAAGGGTATCCAACAATCTTCCCAAGGTCC110.10577940186556399No Hit
TTGAAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCCTG110.10577940186556399No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTCT110.10577940186556399No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)