FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004970908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13552
Sequences flagged as poor quality0
Sequence length86-32863
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAAT6434.7446871310507674No Hit
CCTGAAGAAATGTCACGGCTTGTTGGCGCAGGCCGCCTGCTCTCAGGAAT2802.066115702479339No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGTAGGGAACTTGGC1781.3134592680047226No Hit
CTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAATC1441.062573789846517No Hit
GCCATGACCATTGTTATTTGTCAGAGGTCAAAATTGTAGGGAACTTGGCC950.7010035419126328No Hit
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAAGGAA650.4796340023612751No Hit
CTGAAGAAATGTCACGGCTTGTTGGCGCAGGCCGCCTGCTCTCAGGAATC610.45011806375442737No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGTGAGAACTTGGCC460.3394332939787485No Hit
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGAATC450.3320543093270366No Hit
GCCATGACCATTATTTGTCCAGAGGTCAAAATTGTAGGGAACTTGGCCAC430.3172963400236128No Hit
GCCATGACCATTGTTGTCCAGAGGTCAAAATTGTAGGGAACTTGGCCACC390.28778040141676503No Hit
CCCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAA380.2804014167650531No Hit
GCCATGACCATTATTATTTGTCCAGAGGTCAAAATTGTAGGGAACTTGGC340.25088547815820544No Hit
CCTGAAGAAATGTCACGGCATTGGCGCAGGCCGCCTGCTCTCAGGAATCT330.2435064935064935No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGTAGGAACTTGGCC310.22874852420306963No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGTAGAACTTGGCCA300.22136953955135774No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGGCAGGGAACTTGG290.21399055489964583No Hit
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCCTCAGGAA250.18447461629279813No Hit
CCTGAAGAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAATC230.16971664698937425No Hit
CCTGAAGAAATGTCACGGCTTGTTGGCGCAGGCCGCCTGCTCTCAAGGAA210.15495867768595042No Hit
CCTGAAGAAATGTCGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAATCT200.14757969303423848No Hit
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCAGGAATCT170.12544273907910272No Hit
GCCATGACCATTGTTATTTGTCAGAGGTCAAAATTGTGAGAACTTGGCCA170.12544273907910272No Hit
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCGCCTGCTCTCAGGAATC170.12544273907910272No Hit
CCTGAAGGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAA160.11806375442739078No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGGCAGAACTTGGCC160.11806375442739078No Hit
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGACCGCCTGCTCTCAGGAAT160.11806375442739078No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGGCAGGAACTTGGC150.11068476977567887No Hit
GCCATGACCATTGTTATTTGTCAGAGGTCAAATTGTAGGGAACTTGGCCA150.11068476977567887No Hit
CCTGAAGAAATGTCACGGCTTATTGGCGCAGGCCGCCTGCTCTCAGGAAT150.11068476977567887No Hit
CCTGAAGAAATGTCACGGCTTGTTAAGCGCAGGCCGCCTGCTCTCAGGAA150.11068476977567887No Hit
CCCTGAAGAAATGTCACGGCTTGTTGGCGCAGGCCGCCTGCTCTCAGGAA140.10330578512396695No Hit
CCTGAAGAAATGTACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAATC140.10330578512396695No Hit
CCTGAAGAAATGTCACGGCTTGTTAGCGCAGGCCCGCCTGCTCTCAGGAA140.10330578512396695No Hit
TGAAGAAATGTCACGGCTTGTTAGCGCAGGCCGCCTGCTCTCAGGAATCT140.10330578512396695No Hit
GCCATGACCATTGTTATTTGTCAGAGGTCAAAATTGGCAGGGAACTTGGC140.10330578512396695No Hit
CCTGAAGAAATGTCACGGCTTGATGGCGCAGGCCGCCTGCTCTCAGGAAT140.10330578512396695No Hit
GCCATGACCATTGTTATTTGTCCAGAGGTCAAAATTGTAGGGGAACTTGG140.10330578512396695No Hit
GCCATGACCATTATTTGTCAGAGGTCAAAATTGTAGGGAACTTGGCCACC140.10330578512396695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)