FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004970909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8865
Sequences flagged as poor quality0
Sequence length62-26122
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC1902.143260011280316No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCT1711.9289340101522845No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAGTTCATGCTCT530.5978567399887197No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGTTCATGCTC460.5188945290468133No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACGAGTTCATGCT380.42865200225606315No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAGCAATCTTCCCAAGGT340.3835307388606881No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCACAGAGTTCATG320.36097010716300054No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAGCAATCTTCCCAAGGTC310.3496897913141568No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACGAGTTCATGCTC300.338409475465313No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTC270.3045685279187817No Hit
AGTAACTGAAAATGCTTCTGAGTGGCTAGGATAACAGATGTGCACCACCA270.3045685279187817No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGT230.25944726452340666No Hit
TTGAAGAGAGGTGACAAAACAAAGGAAATGCAGAGGAGACCTTGACATAA210.23688663282571912No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCCTGAGTTCATGCT180.20304568527918782No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTC180.20304568527918782No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCTGAGTTCATGCT160.18048505358150027No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGTTCATGCTC160.18048505358150027No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCC160.18048505358150027No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAAGTTCATG160.18048505358150027No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCACAGAGTTCATGCT150.1692047377326565No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGGATTCATGCT140.15792442188381275No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGATTCATGCTCT140.15792442188381275No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAAGTTCATGCTC140.15792442188381275No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAAGTTCATGCTC140.15792442188381275No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAGCAATCTTCCCAAGGTCC140.15792442188381275No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGATTCATGCTCTC140.15792442188381275No Hit
AGTAACTGAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT130.146644106034969No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCCAGCAATCTTCCCAAGGTCC130.146644106034969No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAAGTTCATGCT130.146644106034969No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAGCAATCTTCCCAAGGTC130.146644106034969No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGGATTCATGC130.146644106034969No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCCTGAGTTCATGCTC120.1353637901861252No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGTTCATGCTCT110.12408347433728144No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCTGAGTTCATGCTCT110.12408347433728144No Hit
CATTGTACTTCGTTCAGTTACGTATTGCTGGTGCTGCCGATCCTTGTGGC110.12408347433728144No Hit
AGTAACTGAAAATGCTTCTGATGGCAGACAGCTGTCACAGAGTTCATGCT110.12408347433728144No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTC100.1128031584884377No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAAGTTCATGC100.1128031584884377No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCC90.10152284263959391No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAAGTTCATGCT90.10152284263959391No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGAGTTCATGC90.10152284263959391No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTCC90.10152284263959391No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAAGGTC90.10152284263959391No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCACGAGTTCATGC90.10152284263959391No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGGGTTCATGCT90.10152284263959391No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)