FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004970910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10227
Sequences flagged as poor quality0
Sequence length36-35443
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC5084.96724357093967No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCT950.9289136599198201No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCACAGAGTTCATGCT790.7724650435122714No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCACAGAGTTCATG660.6453505426811381No Hit
AGTAACTGAAAATGCTTCTGAGTGGCTAGGATAACAGATGTGCACCACCA550.5377921189009485No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGT530.5182360418500049No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTC530.5182360418500049No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTC430.42045565659528694No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCC430.42045565659528694No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGTTCATGCTC390.3813435024933998No Hit
AGTAACTGAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT370.36178742544245623No Hit
TTGAAGAGAGGTGACAAAACAAAGGAAATGCAGAGGAGACCTTGACATAA350.34223134839151265No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATAC330.32267527134056906No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGGTTCATGCT270.26400704018773835No Hit
AGTAACTGAAAATGCTTCCTGAGTGGCAGACAGCTGTCACAGAGTTCATG230.22489488608585118No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCT220.21511684756037938No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTC210.20533880903490762No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACGAGTTCATGCT200.19556077050943582No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCC200.19556077050943582No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTCCT190.185782731983964No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCCAACAATCTTCCCAAGGTCC180.1760046934584922No Hit
AGTAACTGAAAATGCTTCACAGTGGCAGACAGCTGTCACAGAGTTCATGC180.1760046934584922No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACCCAGCTGTCACAGAGTTCAT160.15644861640754865No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTCC160.15644861640754865No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAAGGTC160.15644861640754865No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAAGGT150.14667057788207685No Hit
TTGAAGAGGTGACAAAACAAAGGAAATGCAGAGGAGACCTTGACATAAAG140.13689253935660506No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCCTG140.13689253935660506No Hit
TTGAAGAGAGGTGACAAAACAAAGAAATGCAGAGGAGACCTTGACATAAA130.12711450083113326No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCCATG120.11733646230566148No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCAACAATCTTCCCAAGGTCCT120.11733646230566148No Hit
AGTAGCTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC120.11733646230566148No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAAGAGTTCATG120.11733646230566148No Hit
AGTAACTGAAAATGCTTCTGATGGCAGACAGCTGTCACAGAGTTCATGCT120.11733646230566148No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAAGGT120.11733646230566148No Hit
TTGAAGAGGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTC110.10755842378018969No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAAGG110.10755842378018969No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAAGTTCATGCT110.10755842378018969No Hit
TTGAAGAGAGGTGACAAAACAAAGAGGAGTATCAACAATCTTCCCAAGGT110.10755842378018969No Hit
AGTAACTGAAAATGCTTCTGAGAGTGGCAGACAGCTGTCACAGAGTTCAT110.10755842378018969No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)