FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004970923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9921
Sequences flagged as poor quality0
Sequence length46-25888
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC3073.0944461243826225No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCT1621.6328999092833383No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGT710.7156536639451668No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGTTCATGCTC640.6450962604576151No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTC570.5745388569700636No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTC450.4535833081342607No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACGAGTTCATGCT440.4435036790646104No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAGTTCATGCTCT420.42334442092530994No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTC360.3628666465074085No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCC350.3527870174377583No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCACAGAGTTCATG330.3326277592984578No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCACAGAGTTCATGCT320.3225481302288076No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACGAGTTCATGCTC290.29230924301985683No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTCC280.2822296139502066No Hit
AGTAACTGAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT220.2217518395323052No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCCAACAATCTTCCCAAGGTCC210.21167221046265497No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCT210.21167221046265497No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCCTG210.21167221046265497No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCCTGAGTTCATGCT200.20159258139300476No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTCCT200.20159258139300476No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAAGGT180.18143332325370426No Hit
TTGAAGAGAGGTGACAAAACAAAGGGTATCCAACAATCTTCCCAAGGTCC180.18143332325370426No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTCCT170.17135369418405402No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAAGG170.17135369418405402No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAAGTTCATGCT170.17135369418405402No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGGATTCATGC170.17135369418405402No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCAACAATCTTCCCAAGGTCTG160.1612740651144038No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTCTG160.1612740651144038No Hit
GGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC160.1612740651144038No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCCTGAGTTCATGCTC150.15119443604475355No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAAGGT150.15119443604475355No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGATTCATGCTCT140.1411148069751033No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGTTCATGCTC140.1411148069751033No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAAGGTC140.1411148069751033No Hit
TTGAAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTCCT140.1411148069751033No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTCTG120.12095554883580284No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCCAACAATCTTCCCAAGGTCT120.12095554883580284No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTCT120.12095554883580284No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATAC120.12095554883580284No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGGTTCATGCT110.1108759197661526No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCT110.1108759197661526No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAAGGTC110.1108759197661526No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCAACAATCTTCCCAAGGTCCT110.1108759197661526No Hit
AGTAACTGAAAATGCTTCTGAGTGGCTAGGATAACAGATGTGCACCACCA110.1108759197661526No Hit
TTGAAGAGAGGTGACAAAACAAAGGGTATCAACAATCTTCCCAAGGTCCT100.10079629069650238No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCC100.10079629069650238No Hit
AGTAGCTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC100.10079629069650238No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAAGTTCATG100.10079629069650238No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAAGGTCC100.10079629069650238No Hit
TTGAAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTCTG100.10079629069650238No Hit
TTGAAGAGAGGTGACAAAACAAAGGGGAGTATCAACAATCTTCCCAAGGT100.10079629069650238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)