FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004970924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13695
Sequences flagged as poor quality0
Sequence length40-25557
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCT3302.4096385542168677No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC2752.0080321285140563No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGTTCATGCTC760.5549470609711573No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAGTTCATGCTCT730.5330412559328223No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAGCAATCTTCCCAAGGT640.4673238408178167No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAGCAATCTTCCCAAGGTC540.39430449069003287No Hit
AGTAACTGAAAATGCTTCTGAGTGGCTAGGATAACAGATGTGCACCACCA500.36509675063891933No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTC480.35049288061336253No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGT460.3358890105878058No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCCTGAGTTCATGCTC450.32858707557502737No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACGAGTTCATGCTC420.30668127053669225No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAGCAATCTTCCCAAGGTC400.29207740051113545No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTC390.284775465498357No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACGAGTTCATGCT350.2555677254472435No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCACAGAGTTCATGCT300.21905805038335158No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCC300.21905805038335158No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCACAGAGTTCATG270.19715224534501644No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCCTGAGTTCATGCT260.18985031033223804No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAGCAATCTTCCCAAGGTC260.18985031033223804No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGTTCATGCTCT250.18254837531945967No Hit
AGTAACTGAAAATGCTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCTC230.1679445052939029No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAGCAATCTTCCCAAGGTCC220.1606425702811245No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGTTCATGCTC190.13873676524278933No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCCAGCAATCTTCCCAAGGTCC190.13873676524278933No Hit
AGTAACTGAAAATGCTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT190.13873676524278933No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTC180.13143483023001096No Hit
AGTAACTGAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCTC180.13143483023001096No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAGCAATCTTCCCAAGGTCCT180.13143483023001096No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAGCAATCTTCCCAAGGTCC180.13143483023001096No Hit
AGTAACTGAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT160.11683096020445417No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCC160.11683096020445417No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTCCT160.11683096020445417No Hit
AGTAACTGAAAATGCTTCTAATTGGCAGACAGCTGTCACAGAGTTCATGC150.10952902519167579No Hit
AGTAACTGAAAATGCTTCTAATTAGCTAGGATAACAGATGTGCACCACCA140.1022270901788974No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGATTCATGCTCTC140.1022270901788974No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATACT140.1022270901788974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)