FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004970927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10316
Sequences flagged as poor quality0
Sequence length11-25469
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC3543.4315626211709964No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCT1681.6285381930980998No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGTTCATGCTC990.9596742923613804No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGT790.76580069794494No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTC780.7561070182241179No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTC740.7173322993408298No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACGAGTTCATGCT530.5137650252035673No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCC490.47499030632027917No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCACAGAGTTCATG490.47499030632027917No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAGTTCATGCTCT440.4265219077161691No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCCTGAGTTCATGCT410.397440868553703No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCACAGAGTTCATGCT410.397440868553703No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTC390.3780535091120589No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGTTCATGCTC350.33927879022877083No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACGAGTTCATGCTC250.2423419930205506No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCT240.23264831329972857No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCCTG240.23264831329972857No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTCCT230.22295463357890655No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCCAACAATCTTCCCAAGGTCC230.22295463357890655No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAAGGT230.22295463357890655No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAAGGTC220.21326095385808455No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCC210.20356727413726247No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAAGG210.20356727413726247No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCAACAATCTTCCCAAGGTCCT190.18417991469561845No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTCCT180.17448623497479643No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCCTGAGTTCATGCTC170.1647925552539744No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATAC170.1647925552539744No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGGTTCATGCT160.15509887553315238No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGAGTTCATGC160.15509887553315238No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTCC160.15509887553315238No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAAGGT150.14540519581233036No Hit
TTGAAGAGAGGTGACAAAACAAAGGAAATGCAGAGGAGACCTTGACATAA150.14540519581233036No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGTTCATGCTCT140.13571151609150833No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATACT140.13571151609150833No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGATTCATGCTCT120.11632415664986429No Hit
TTGAAGAGGTGACAAAACAAGGAGTATCCAACAATCTTCCCAAGGTCCTG120.11632415664986429No Hit
TTGAAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCCTG120.11632415664986429No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTCTG120.11632415664986429No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCT110.10663047692904228No Hit
AGTAACTGAAAATGCTTCTGATGGCAGACAGCTGTCACAGAGTTCATGCT110.10663047692904228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)