FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004970931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11554
Sequences flagged as poor quality0
Sequence length66-24734
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC2932.5359182966937857No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCT2251.9473775315907909No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAGTTCATGCTCT820.7097109226241993No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGT750.6491258438635971No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTC710.6145057988575385No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGTTCATGCTC680.5885407651029947No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACGAGTTCATGCT610.5279556863423922No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTC540.46737060758178983No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACGAGTTCATGCTC490.42409555132421667No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTC470.4067855288211875No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCC450.3894755063181582No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCCTGAGTTCATGCT350.3029253938030119No Hit
TTGAAGAGAGGTGACAAAACAAAGGGGAGTATCAACAATCTTCCCAAGGT340.29427038255149734No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCACAGAGTTCATG290.2509953262939242No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAAGGT280.24234031504240958No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCCTGAGTTCATGCTC280.24234031504240958No Hit
TTGAAGAGAGGTGACAAAACAAAGGGGAGTATCCAACAATCTTCCCAAGG260.22503029253938028No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTCC260.22503029253938028No Hit
TTGAAGAGAGGTGACAAAACAAGGGGAGTATCCAACAATCTTCCCAAGGT250.21637528128786568No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCT240.20772027003635105No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCACAGAGTTCATGCT230.1990652587848364No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCC230.1990652587848364No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGTTCATGCTC210.18175523628180718No Hit
TTGAAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCCTG200.17310022503029254No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGTTCATGCTCT190.1644452137787779No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGATTCATGCTCT170.14713519127574867No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAAGGT160.138480180024234No Hit
AGTAACTGAAAATGCTTCTAATTGGCAGACAGCTGTCACAGAGTTCATGC160.138480180024234No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTCCT160.138480180024234No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAAGGT160.138480180024234No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTCCT150.1298251687727194No Hit
AGTAACTGAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT150.1298251687727194No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAAGG150.1298251687727194No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGATTCATGCTCTC150.1298251687727194No Hit
AGTAACTGAAAATGCTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT150.1298251687727194No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATACT150.1298251687727194No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCAACAATCTTCCCAAGGTCTG140.12117015752120479No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCT140.12117015752120479No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTCT140.12117015752120479No Hit
TTGAAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTCCT140.12117015752120479No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAAGGTC130.11251514626969014No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCCAACAATCTTCCCAAGGTCC130.11251514626969014No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTCTG120.10386013501817552No Hit
AGTAACTGAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCTC120.10386013501817552No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAAGTTCATGCT120.10386013501817552No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATAC120.10386013501817552No Hit
AGTAACTGAAAATGCTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCTC120.10386013501817552No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)