FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004970932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15568
Sequences flagged as poor quality0
Sequence length60-26065
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC4552.922661870503597No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCT2611.6765159301130526No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAGCAATCTTCCCAAGGTC1450.9313977389516958No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGTTCATGCTC1280.8221993833504625No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAGCAATCTTCCCAAGGT1250.802929085303186No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAGCAATCTTCCCAAGGTC750.48175745118191166No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACGAGTTCATGCT730.46891058581706063No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAGCAATCTTCCCAAGGTC720.4624871531346352No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAGTTCATGCTCT660.4239465570400823No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAGCAATCTTCCCAAGGTCC610.39182939362795477No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCACAGAGTTCATG580.3725590955806783No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACGAGTTCATGCTC440.28263103802672146No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCCAGCAATCTTCCCAAGGTCC410.26336073997944504No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAGCAATCTTCCCAAGGTCC390.250513874614594No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAGCAATCTTCCCAAGGTCCT370.23766700924974307No Hit
TTGAAGAGGTGACAAAACAAGGGAGTATCCAGCAATCTTCCCAAGGTCCT340.21839671120246656No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCCTGAGTTCATGCT330.21197327852004114No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAGCAATCTTCCCAAAGGT330.21197327852004114No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCACAGAGTTCATGCT330.21197327852004114No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCAGCAATCTTCCCAAGGTCCT300.19270298047276466No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGTTCATGCTC290.18627954779033915No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGTTCATGCTCT280.1798561151079137No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAGCAATCTTCCCAAAGG260.1670092497430627No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCCTGAGTTCATGCTC240.1541623843782117No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAGCAATCTTCCCAAGGTCC230.14773895169578624No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAGCAATCTTCCCAAGGTCTG220.14131551901336073No Hit
TTGAAGAGGTGACAAAACAAGGAGTATCCAGCAATCTTCCCAAGGTCCTG210.13489208633093525No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCAGCAATCTTCCCAAGGTCCTG190.12204522096608428No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAGCAATCTTCCCAAAGGTC190.12204522096608428No Hit
AGTAACTGAAAATGCTTCTGATGGCAGACAGCTGTCACAGAGTTCATGCT190.12204522096608428No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCAGCAATCTTCCCAAGGTCCT190.12204522096608428No Hit
AGTAACTGAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT180.1156217882836588No Hit
AGTAACTGAAAATGCTTCTGAGTGGCTAGGATAACAGATGTGCACCACCA180.1156217882836588No Hit
TTGAAGAGAGGTGACAAAACAAAGGGTATCCAGCAATCTTCCCAAGGTCC170.10919835560123328No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCTGAGTTCATGCT170.10919835560123328No Hit
TTGAAGAGAGGTGACAAAACAAAGGGGAGTATCCAGCAATCTTCCCAAGG170.10919835560123328No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAGCAATCTTCCCAAGGTCT170.10919835560123328No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATACT170.10919835560123328No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAGCAATCTTCCCAAGGTCT170.10919835560123328No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATAC160.10277492291880781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)