FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004970933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13970
Sequences flagged as poor quality0
Sequence length19-29432
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC3312.369362920544023No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCT2531.811023622047244No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAGCAATCTTCCCAAGGT1100.7874015748031495No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAGCAATCTTCCCAAGGTC850.6084466714387974No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGTTCATGCTC840.6012884753042234No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAGCAATCTTCCCAAGGTC670.4795991410164639No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAGTTCATGCTCT620.4438081603435934No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAGCAATCTTCCCAAGGTC570.40801717967072304No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAGCAATCTTCCCAAGGTCC450.322118826055834No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACGAGTTCATGCT420.3006442376521117No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAGCAATCTTCCCAAGGTCC380.2720114531138153No Hit
CGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC360.25769506084466715No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTC360.25769506084466715No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACGAGTTCATGCTC340.24337866857551899No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGGATTCATGC340.24337866857551899No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCACAGAGTTCATGCT320.2290622763063708No Hit
TTGAAGAGAGGTGACAAAACAAAGGGGAGTATCCAGCAATCTTCCCAAGG320.2290622763063708No Hit
TTGAAGAGGTGACAAAACAAGGGAGTATCCAGCAATCTTCCCAAGGTCCT300.21474588403722264No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCACAGAGTTCATG290.20758768790264853No Hit
TTGAAGAGAGGTGACAAAACAAGGGGAGTATCCAGCAATCTTCCCAAGGT290.20758768790264853No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGT260.18611309949892627No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGTTCATGCTC250.17895490336435219No Hit
CGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCT240.1717967072297781No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTC230.164638511095204No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCCTGAGTTCATGCTC230.164638511095204No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCCTGAGTTCATGCT220.15748031496062992No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAGCAATCTTCCCAAAGG220.15748031496062992No Hit
TTGAAGAGGTGACAAAACAAAGGGGAGTATCCAGCAATCTTCCCAAGGTC220.15748031496062992No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGTTCATGCTCT220.15748031496062992No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCC190.13600572655690765No Hit
TTGAAGAGAGGTGACAAAACAAGGGGAGTATCAGCAATCTTCCCAAGGTC180.12884753042233357No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAGCAATCTTCCCAAGGTCT180.12884753042233357No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAGAGTTCATGCT170.12168933428775949No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAAGTTCATG170.12168933428775949No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAGCAATCTTCCCAAGGTCC170.12168933428775949No Hit
TTGAAGAGAGGTGACAAAACAAAGGGGAGTATCAGCAATCTTCCCAAGGT170.12168933428775949No Hit
TTGAAGAGAGGTGACAAAACAAAGGGGAGTATCCAGCAATCTTCCCAAAG160.1145311381531854No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCCAGCAATCTTCCCAAGGTCC160.1145311381531854No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAGCAATCTTCCCAAGGTCT160.1145311381531854No Hit
TTGAAGAGGTGACAAAACAAGGGAGTATCAGCAATCTTCCCAAGGTCCTG140.10021474588403723No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGATTCATGCTCT140.10021474588403723No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAAGTTCATGCT140.10021474588403723No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGATTCATGCTCTC140.10021474588403723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)