FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004970938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20577
Sequences flagged as poor quality0
Sequence length4-31392
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC7713.7469018807406327No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCT6773.290081158575108No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCACAGAGTTCATG1670.8115857510813044No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTC1280.6220537493317783No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGT1180.573455800165233No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTC990.4811196967487972No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCC920.4471011323322156No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAGTTCATGCTCT830.4033629780823249No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTC670.32560625941585264No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGTTCATGCTC650.31588666958254363No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCACAGAGTTCATGCT570.2770083102493075No Hit
AGTAACTGAAAATGCTTCTGAGTGGCTAGGATAACAGATGTGCACCACCA460.2235505661661078No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAAGTTCATGCTC440.21383097633279877No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAAGAGTTCATGC440.21383097633279877No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTAGAGTTCATGCT430.20897118141614424No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCT410.19925159158283523No Hit
AGTAACTGAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT410.19925159158283523No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAAGAGTTCATG410.19925159158283523No Hit
TTGAAGAGAGGTGACAAAACAAAGGAAATGCAGAGGAGACCTTGACATAA380.18467220683287164No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAAGTTCATGCTC380.18467220683287164No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTCCT370.17981241191621714No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTCC370.17981241191621714No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCCTG360.17495261699956263No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAAGGTC350.1700928220829081No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCCAACAATCTTCCCAAGGTCC340.16523302716625357No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAAGTTCATGCT330.16037323224959907No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTCCT320.15551343733294457No Hit
AGTAACTGAAAATGCTTCTGATGGCAGACAGCTGTCACAGAGTTCATGCT310.15065364241629003No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAAGG310.15065364241629003No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTAGAGTTCATGCTC310.15065364241629003No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATACT290.140934052582981No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCC280.13607425766632647No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAAGTTCATGCT260.12635466783301746No Hit
AGTAACTGAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCTC250.12149487291636292No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGGTTCATGCT250.12149487291636292No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTCTG240.1166350779997084No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCCAACAATCTTCCCAAGGTCT230.1117752830830539No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAAGGT230.1117752830830539No Hit
AGTAACTGAAAATGCTTCACAGTGGCAGACAGCTGTCACAGAGTTCATGC220.10691548816639938No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATAC210.10205569324974485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)