FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004970939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25001
Sequences flagged as poor quality0
Sequence length50-25953
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC10164.0638374465021405No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCT3421.3679452821887124No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGTTCATGCTC2751.0999560017599295No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACGAGTTCATGCT1810.7239710411583536No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAGCAATCTTCCCAAGGT1710.6839726410943562No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAGCAATCTTCCCAAGGTC1650.6599736010559578No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGT840.3359865605375785No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTC840.3359865605375785No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCCTGAGTTCATGCT790.31598736050557974No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAGCAATCTTCCCAAGGTC770.3079876804927803No Hit
AGTAACTGAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT760.30398784048638056No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGTTCATGCTC740.29598816047358106No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAGTTCATGCTCT730.2919883204671813No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCACAGAGTTCATGCT710.2839886404543818No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTC680.2719891204351826No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACGAGTTCATGCTC670.26798928042878284No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAGCAATCTTCCCAAGGTCC610.2439902403903844No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAGCAATCTTCCCAAGGTC590.2359905603775849No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCACAGAGTTCATG580.23199072037118515No Hit
AGTAACTGAAAATGCTTCTGAGTGGCTAGGATAACAGATGTGCACCACCA470.18799248030078797No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCC460.18399264029438822No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAGCAATCTTCCCAAGGTCC460.18399264029438822No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATAC440.17599296028158873No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGGATTCATGC430.171993120275189No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAGCAATCTTCCCAAAGG370.14799408023679053No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCCAGCAATCTTCCCAAGGTCC360.14399424023039079No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAGCAATCTTCCCAAAGGT350.13999440022399104No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGATTCATGCTCT350.13999440022399104No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTCC340.1359945602175913No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTC330.13199472021119155No Hit
TTGAAGAGGTGACAAAACAAAGGAGTATCCAGCAATCTTCCCAAGGTCCT320.1279948802047918No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAAGTTCATGCT320.1279948802047918No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGAGTTCATGC320.1279948802047918No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCCTGAGTTCATGCTC310.12399504019839207No Hit
AGTAACTGAAAATGCTTCTGATGGCAGACAGCTGTCACAGAGTTCATGCT280.11199552017919284No Hit
AGTAGCTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC270.1079956801727931No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCT270.1079956801727931No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGTTCATGCTCT260.10399584016639334No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCTGAGTTCATGCTCT260.10399584016639334No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)