FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004970940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004970940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11978
Sequences flagged as poor quality0
Sequence length36-25993
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGC3903.255969277007847No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAGTTCATGCT2241.8700951744865586No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGT770.6428452162297545No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTC750.6261479378861246No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGTTCATGCTC710.5927533811988646No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTC620.5176156286525296No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAGTTCATGCTCT580.48422107196526964No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACGAGTTCATGCT550.45917515444982465No Hit
TTGAAGAGAGGTGACAAAACAAAGGGGAGTATCAACAATCTTCCCAAGGT400.33394556687259974No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCC330.2755050926698948No Hit
AGTAACTGAAAATGCTTCTGAAGTGGCAGACAGCTGTCACAGAGTTCATG300.2504591751544498No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGTTCATGCTC290.24211053598263482No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGGATTCATGC290.24211053598263482No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCCTGAGTTCATGCT270.22541325763900483No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTCC270.22541325763900483No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCCAACAATCTTCCCAAGGTC270.22541325763900483No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCCTGAGTTCATGCTC240.20036734012355986No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAAGTTCATG240.20036734012355986No Hit
TTGAAGAGAGGTGACAAAACAAAGGGGAGTATCCAACAATCTTCCCAAGG230.19201870095174486No Hit
TTGAAGAGAGGTGACAAAACAAGGGGAGTATCAACAATCTTCCCAAGGTC230.19201870095174486No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACGAGTTCATGCTC220.18367006177992987No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGATTCATGCTCT220.18367006177992987No Hit
TTGAAGAGAGGTGACAAAACAAGGGGAGTATCCAACAATCTTCCCAAGGT220.18367006177992987No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAGGTCCT210.17532142260811487No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGTTCATGCTCT200.16697278343629987No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAAGTTCATGCT200.16697278343629987No Hit
AGTAACTGAAAATGCTTCTGAGTGGCTAGGATAACAGATGTGCACCACCA200.16697278343629987No Hit
TTGAAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAGGTCCT190.1586241442644849No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAAGG180.1502755050926699No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCC180.1502755050926699No Hit
AGTAACTGAAAATGCTTCTGGTGGCAGACAGCTGTCACAGAGTTCATGCT170.1419268659208549No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAGGTCT170.1419268659208549No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAAGTTCATGCT170.1419268659208549No Hit
AGTAACTGAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATGCT160.1335782267490399No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAAGGTC160.1335782267490399No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCACAGAAGTTCATGC160.1335782267490399No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCTGAGAGTTCATGC150.1252295875772249No Hit
TTGAAGAGGTGACAAAACAAGGGAGTATCAACAATCTTCCCAAGGTCCTG150.1252295875772249No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGATTCATGCTCTC150.1252295875772249No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCAACAATCTTCCCAAGGTCCT140.11688094840540991No Hit
TTGAAGAGAGGTGACAAAACAAAGGAGTATCAACAATCTTCCCAAAGGTC140.11688094840540991No Hit
TTGAAGAGAGGTGACAAAACAAGGGAGTATCCAACAATCTTCCCAAAGGT130.10853230923359493No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAAATGCAGAGGAGACCTTGACATA130.10853230923359493No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACGATTCATGCTC130.10853230923359493No Hit
TTGAAGAGGTGACAAAACAAAGGGAGTATCCAACAATCTTCCCAAGGTCT130.10853230923359493No Hit
TTGAAGAGGTGACAAAACAAAGGGGAGTATCCAACAATCTTCCCAAGGTC130.10853230923359493No Hit
TTGAAGAGAGGTGACAAAACAAGGAGTATCCAACAATCTTCCCAAGGTCC120.10018367006177993No Hit
TTGAAGAGAGGTGACAAAACAAAGGGAGTATCAACAATCTTCCCAAAGGT120.10018367006177993No Hit
AGTAACTGAAAATGCTTCTAGTGGCAGACAGCTGTCTGAAGTTCATGCTC120.10018367006177993No Hit
AGTAACTGAAAATGCTTCTGAGTGGCAGACAGCTGTCACAGAGTTCATAC120.10018367006177993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)